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Cl316 243 disodium salt

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CL316,243 disodium salt is a selective β3-adrenergic receptor agonist. It stimulates the β3-adrenergic receptor, which is involved in the regulation of energy metabolism and thermogenesis. The product is intended for research use only and is not for use in diagnostic procedures.

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Lab products found in correlation

Isoproterenol hydrochloride, by Merck Group (2 mentions) Mirabegron, by Cayman Chemical (1 mentions) Nadolol, by Merck Group (1 mentions) R phenylephrine hydrochloride, by Bio-Techne (1 mentions) Dexmedetomidine hydrochloride, by Bio-Techne (1 mentions) Rs79948 hydrochloride, by Bio-Techne (1 mentions) Ici 118 551 hydrochloride, by Bio-Techne (1 mentions) Rosiglitazone, by Santa Cruz Biotechnology (1 mentions) Isobutylmethylxanthine, by Merck Group (1 mentions) Gsk3787, by Selleck Chemicals (1 mentions) Gw9662, by Selleck Chemicals (1 mentions) T0070907, by Selleck Chemicals (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) P ampkα thr172, by Cell Signaling Technology (1 mentions) Ps6 ser235 236, by Cell Signaling Technology (1 mentions) P akt thr308, by Cell Signaling Technology (1 mentions) Tween 80, by Selleck Chemicals (1 mentions) P eif2α ser51, by Cell Signaling Technology (1 mentions)

5 protocols using cl316 243 disodium salt

1

Adrenergic Receptor Agonists: In Vivo, In Vitro, and Ex Vivo Applications

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The pan β-adrenergic agonist, isoproterenol hydrochloride
(ISO) was from Sigma; the β3-adrenoreceptor agonists, CL
316,243 disodium salt (CL) was obtained from Tocris Bioscience (UK)
and mirabegron (Mira) was from Cayman Chemical. For CL
administration in vivo, mice were acclimated to
thermoneutrality for 3 days, injected with 1 mg/kg CL, and euthanized for tissue
collection 60 minutes later. For β-adrenergic stimulation in
vitro, 3T3-L1 cells were treated with ISO (10 μM) for 40
minutes at 370C. For β-adrenergic stimulation ex
vivo, human fat samples (approx. 0.5cm in size) were treated with
ISO (10 μM) for 40 minutes at 370C. For β-adrenergic
stimulation in vivo, human subjects were administered Mira for
10 weeks (50 mg/day), with abdominal adipose biopsies before and after
treatment, as described previously (Finlin
et al., 2018).
Kasza I., Kühn J.P., Völzke H., Hernando D., Xu Y.G., Siebert J.W., Gibson A.L., Yen C.L., Nelson D.W., MacDougald O.A., Richardson N.E., Lamming D.W., Kern P.A, & Alexander C. (2021). Contrasting recruitment of skin-associated adipose depots during cold challenge of mouse and human. The Journal of physiology, 600(4), 847-868.
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2

Modulation of B cell activation by adrenergic signals

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For in vitro stimulation with ADR ligands, splenic B cells were seeded at 2.5 × 105 cells per well in a 96-well plate. B cells were either incubated with the pan ß-ADR antagonist nadolol [5 μM, Sigma-Aldrich] or the indicated concentrations of α-and ß-ADR antagonists 30 min prior to activation followed by treatment with 5 μg/mL anti-IgM and 1.25 μg/mL CpG or were first activated with anti-IgM/CpG for 4 h followed by incubation with indicated concentrations of α- or ß-ADR agonists for 24 h. (Agonists: ADRA1: (R)-(-)-Phenylephrine hydrochloride (Tocris, Bristol, UK, catalog number: 2838), ADRA2: Dexmedetomidine hydrochloride (Tocris, catalog number: 2749), ADRB2: L-(-)—NE (+)-biartrate salt monohydrate (Sigma-Aldrich, catalog number: A9512), ADRB3: CL316243 disodium salt (Tocris, catalog number: 1499), ADRB: (-)-Isoproterenol hydrochloride (Sigma-Aldrich, catalog number: I6504). Antagonists: ADRA2: RS79948 hydrochloride (Tocris, catalog number: 0987), ADRB2: ICI 118,551 hydrochloride (Tocris, catalog number: 0821).
Honke N., Lowin T., Opgenoorth B., Shaabani N., Lautwein A., Teijaro J.R., Schneider M, & Pongratz G. (2022). Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells. PLoS Biology, 20(1), e3001513.
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3

CL-316,243 Disodium Salt Treatment

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CL-316,243 disodium salt (1499, Tocris Biosciences) was administered systemically by daily intraperitoneal injection at a dose of 1 mg kg−1 in PBS. After 21 days of treatment, the mice were euthanized for further analysis.
Seki T., Yang Y., Sun X., Lim S., Xie S., Guo Z., Xiong W., Kuroda M., Sakaue H., Hosaka K., Jing X., Yoshihara M., Qu L., Li X., Chen Y, & Cao Y. (2022). Brown-fat-mediated tumour suppression by cold-altered global metabolism. Nature, 608(7922), 421-428.
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4

Phosphatase Inhibitor Library Evaluation

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The Phosphotase Inhibitor Library was obtained from the National Compound Resource Center (NCRC, Shanghai). Other reagents were obtained as indicated: BML-260 (Life Chemicals, F0207-0150), ISO (Sigma, I6379), CL316243 disodium salt (Tocris, 1499), GW9662 (Selleck, S2915), T0070907(Selleck, S2871), GSK3787(Selleck, S8025), insulin (Sigma, I3536), T3 (Sigma, T2877), indomethacin (Sigma, I7378), dexamethasone (Sigma, D4902), isobutylmethylxanthine (Sigma, I5879), and rosiglitazone (Santa Cruz, sc202795).
Feng Z., Wei Y., Zhang Y., Qiu Y., Liu X., Su L., Liang N., Yin H, & Ding Q. (2019). Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression. Theranostics, 9(12), 3501-3514.
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5

Evaluating Thermogenic Regulators in Adipocytes

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CL316,243 disodium salt (CL), a selective β3-adrenoceptor agonist, and SR59230A hydrochloride (SR), a selective β3 adrenoceptor antagonist, were purchased from TOCRIS. Rapamycin was obtained from AdipoGen Life Sciences (Cat#: AG-CN2-0025, San Diego, CA). Tween 80 (Cat#: S6702) and PEG300 (Cat#: S6704) were both from SELLECKCHEM (Radnor, PA). DMSO was from Sigma Aldrich (Cat#: D8418, Saint Louis, MO). The antibody for β-actin was from ABclonal (Cat#: AC026, Woburn, MA) and antibody for PGC1α was from NOVUS Biologicals (Cat#: NBP1-04676, Centennial, CO). The following antibodies were from Cell Signaling Biotechnology (Danvers, MA): p-S6 (Ser 235/236) (Cat#: 4858S), S6 (Cat#: 2217S), p-S6K1 (Thr389) (Cat#: 9205S), p-Akt (Thr 308) (Cat#: 4056S), Akt (Cat#: 9272S), p-AMPKα (Thr 172) (Cat#: 2535T), AMPKα (Cat#: 5832T), UCP1 (Cat#: 14670S), and p-eIF2α (Ser 51) (Cat#: 3398T).
Song Q., Chen Y., Ding Q., Griffiths A., Liu L., Park J., Liew C.W., Nieto N., Li S., Dou X., Jiang Y, & Song Z. (2023). mTORC1 inhibition uncouples lipolysis and thermogenesis in white adipose tissue to contribute to alcoholic liver disease. Hepatology Communications, 7(3), e0059.
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