For MTT assay, HCCLM3 cells overexpressing miR-3653 or those in the control group, and Hep3B cells with miR-3653 knockdown or those in the negative control group (5,000 cells/well) were seeded into 96-well plates. Twenty-four, 28 and 72 h after cell seeding, MTT (Sigma-Aldrich; Merck KGaA) was added into each well and incubation was carried out for 4 h at 37°C. Absorbance at 490 nm was measured as the indicator of cell viability. For BrdU assay, HCC cells described above were stained with bromodeoxyuridine for 1 h at room temperature and then incubated with anti-BrdUrd antibody (Sigma-Aldrich; Merck KGaA). BrdU-positive cells were counted under fluorescence microscope (Axioskop 2 Plus; Carl Zeiss Co., Ltd., Jena, Germany).
Anti brdurd antibody
Manufactured by Merck Group
Sourced in United States
The Anti-BrdUrd antibody is a laboratory reagent used in cellular and molecular biology research. It is a monoclonal antibody that specifically binds to bromodeoxyuridine (BrdU), a synthetic nucleoside analog of thymidine that is incorporated into the DNA of dividing cells. This antibody can be used to detect and quantify cell proliferation in various experimental systems.
Automatically generated - may contain errors
4 protocols using anti brdurd antibody
1
Cell Viability and Proliferation Assays
2
Bromodeoxyuridine Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells grown on coverslips (Fisher, Pittsburgh, PA, USA) were incubated with bromodeoxyuridine (BrdUrd) for 1 h and stained with anti-BrdUrd antibody (Sigma) according to the manufacturer's instruction. Gray level images were acquired under a laser scanning microscope (Axioskop 2 plus, Carl Zeiss Co. Ltd., Jena, Germany).
3
Bromodeoxyuridine Labeling and Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells grown on coverslips (Fisher, Pittsburgh, PA, USA) were incubated with bromodeoxyuridine (BrdUrd) for 1 h and stained with anti-BrdUrd antibody (Sigma) according to the manufacturer's instruction. Gray level images were acquired under a laser scanning microscope (Axioskop 2 plus, Carl Zeiss Co. Ltd., Jena, Germany). All experiments were performed in triplicate.
4
Cell Proliferation Analysis via BrdUrd Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated on coverslips (Thermo Fisher Scientific, Waltham, MA, USA ) and allowed to settle for 24 hours, incubated with bromodeoxyuridine (BrdUrd) for 1 h and stained with anti-BrdUrd antibody (Sigma) according to the manufacturer’s instruction. Gray level images were acquired under a laser scanning microscope (Nikon Co., Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!