Osmomat 030 d cryoscopic osmometer

Manufactured by Gonotec

Sourced in Germany

The Osmomat 030-D is a cryoscopic osmometer designed for the measurement of osmolality in various liquids. It determines the osmotic pressure of a sample by measuring the freezing point depression. The instrument is capable of performing accurate and reliable osmolality measurements.

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Lab products found in correlation

Bioprofile flex analyzer, by Nova Biomedical (1 mentions) Nanodrop 2000, by Thermo Fisher Scientific (1 mentions) Cedex bio analyzer, by Roche (1 mentions)

Spelling variants of this product

Osmomat 030 (126 citations) Osmomat 030-D (17 citations) Osmomat 030 osmometer (8 citations) Osmomat 030 cryoscopic osmometer (7 citations) Cryoscopic osmometer (2 citations)

5 protocols using osmomat 030 d cryoscopic osmometer

Bioreactor Characterization: Metabolite and IgG Profiles

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Glucose and lactate concentrations were daily measured using an YSI analyzer (YSI 2700, YSI Life Sciences, Yellow Springs, OH, USA). Ammonium concentrations were analyzed by a BioProfile^® FLEX analyzer (Nova biomedical, Waltham, MA, USA) after the experiment was finished. Spent medium composition including all the extracellular amino acids was quantified using NMR (Spinnovation biologics BV, Oss, The Netherlands) based on the sample scheme (Table 1) after the experiment was finished. IgG concentration was determined by Bioceros Netherlands BV, using the Octet system with protein G biosensors (ForteBio, Pall, Portsmouth, UK). Osmolality was measured using an Osmomat 030-D cryoscopic osmometer (Gonotec, Berlin, Germany).Table 1

Sample points for spent media analysis. Samples for spent media analysis were carried out on every other day for each culture. Grey cells: culture was terminated

Day	0	2	4	6	8	10	12	13	14
BC-P
Opti + EFA/B	√	√	√	√	√	√
Opti + Acti A/B	√	√	√	√	√	√	√		√
Acti + EFA/B	√	√	√	√	√	√	√	√
Acti + Acti A/B	√	√	√	√	√	√	√		√
BC-G
Opti + EFA/B	√	√	√	√	√	√	√		√
Opti + Acti A/B	√	√	√	√	√	√	√
Acti + EFA/B	√	√	√	√	√	√	√		√
Acti + Acti A/B	√	√	√	√	√	√	√		√

Pan X., Streefland M., Dalm C., Wijffels R.H, & Martens D.E. (2016). Selection of chemically defined media for CHO cell fed-batch culture processes. Cytotechnology, 69(1), 39-56.

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Measuring Osmolality of Arg·Glu Solutions

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The osmolality of Arg·Glu solutions in the presence and absence of a mAb2 was measured using an Osmomat 030-D Cryoscopic Osmometer (Gonotec GmbH, Berlin, Germany). Measurement results are shown in Supplementary Information, Fig. S1. Arg·Glu concentrations of 5, 10, 25, 50, 100, 150 and 200 mM were prepared from a 1 M stock in MilliQ water, and also at concentrations of 50, 150 and 200 mM in 10 mM C–P buffer, pH 6.0. For solutions containing protein, mAb2 was buffer exchanged using overnight dialysis into 10 mM C–P buffer, pH 6.0, containing Arg·Glu concentrations of 50, 150 and 200 mM. The protein concentration was adjusted by dilution with the appropriate Arg·Glu solution to 30 mg/ml; concentrations were verified in triplicate using a Nano-Drop 2000 (Thermoscientific, Stafford House, Hertfordshire), by measuring optical absorption at 280 nm.

Kheddo P., Tracka M., Armer J., Dearman R.J., Uddin S., van der Walle C.F, & Golovanov A.P. (2014). The effect of arginine glutamate on the stability of monoclonal antibodies in solution. International Journal of Pharmaceutics, 473(1-2), 126-133.

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Hydrogel pH and Osmolarity Measurement

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The pH values of the synthesized gels were obtained using a SensION pH meter (HACH, pH31). Moreover, after removing air bubbles from the synthesized hydrogels, their osmolality was calculated using an Osmomat 030-D cryoscopic osmometer (Gonotec). Distilled water and 300 mOsmol·kg⁻¹ of NaCl/H₂O standard solution were used as blank and calibration solutions, respectively.

Andrade del Olmo J., Pérez-Álvarez L., Sáez Martínez V., Benito Cid S., Pérez González R., Vilas-Vilela J.L, & Alonso J.M. (2022). Drug Delivery from Hyaluronic Acid–BDDE Injectable Hydrogels for Antibacterial and Anti-Inflammatory Applications. Gels, 8(4), 223.

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Fed-Batch Culture Protocol for Mammalian Cell Lines

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The shaking flask fed-batch culture experiments were performed in 250 mL shaking flasks (Cat. No. 431144, Corning) with a 50 mL working volume at a seeding density of 5 × 10⁵ cells/mL. Dynamis medium was used for the fed-batch culture. Cultures were agitated at 120 rpm in an incubator operated with 8% CO₂ at 37°C and 95% humidity. Efficient Feed C + supplement (Cat. No. A1327501, Gibco) solution was added on days 3, 5, 7, and 10 at volumes of 10% of the initial working volume. Temperature was shifted to 33°C when cell concentrations reached 15–20 × 10⁶ cells/mL. Cultures were harvested on day 14 or when viability dropped below 70%. Glucose, lactate, glutamine, and ammonium concentrations were measured daily using a Cedex-Bio analyzer (Roche, Switzerland). Osmolality was measured daily using an Osmomat 030-D cryoscopic osmometer (Gonotec, Berlin, Germany).

Li J., Wei S., Cao C., Chen K., He H, & Gao G. (2019). Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines. Electronic Journal of Biotechnology, 41, 56-59.

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Osmolality Measurement of Amino Acid Salts

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The osmolality of Arg·Glu, NaCl, Arg·HCl and NaGlu solutions was measured using an Osmomat 030-D Cryoscopic Osmometer (Gonotec GmbH, Berlin, Germany) following standard operating procedures. A 1 M stock solution formulated in RPMI media was prepared for each salt and final concentrations of 5, 100, 150 and 200 mM were prepared for each compound for analysis and construction of a standard curve.

Kheddo P., Golovanov A.P., Mellody K.T., Uddin S., van der Walle C.F, & Dearman R.J. (2016). The effects of arginine glutamate, a promising excipient for protein formulation, on cell viability: Comparisons with NaCl. Toxicology in Vitro, 33, 88-98.

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