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Sc 2301

Manufactured by Santa Cruz Biotechnology
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The Sc-2301 is a laboratory instrument designed for the purpose of performing spectroscopic analysis. The core function of this product is to accurately measure and analyze the absorption or emission spectra of various samples.

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Lab products found in correlation

Ab3274, by Merck Group (2 mentions) Ab3553, by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) A4416, by Merck Group (1 mentions) Sc 69879, by Santa Cruz Biotechnology (1 mentions) Immobilon p membrane, by Merck Group (1 mentions) Doxycycline hyclate, by Merck Group (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Ab32072, by Abcam (1 mentions) Ab124721, by Abcam (1 mentions) C digit blot scanner, by LI COR (1 mentions) Transit lt1 reagent, by Mirus Bio (1 mentions) Sc 130657, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions) A3415, by Merck Group (1 mentions)

5 protocols using sc 2301

1

Mouse Lung Fibroblast Protein Analysis

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Lungs from 5- to 8-week-old mice were dissociated in Hank’s Balanced Salt Solution containing 100 mg/mL type II collagenase (Fisher Scientific 10738473) and passed through 70 μm filters to obtain single-cell suspensions. Mouse adult lung fibroblasts (MALFs) were cultured and maintained in DMEM (Thermo Fisher 41966052) supplemented with penicillin-streptomycin (Thermo Fisher, 15140-122), l-glutamine (Thermo Fisher, 25030-024), and FBS (Scientific labs, F7524). For experimental purposes, MALFs were treated with 1 μg/ml doxycycline hyclate (Sigma D9891). Proteins from MALFs were extracted using standard protocols. Total protein lysates from MALFs were electrophoresed on an SDS-PAGE gel and blotted onto Immobilon-P membrane (Millipore). Membranes were blocked with 5% nonfat milk and primary antibodies incubated overnight at 4 °C. Secondary antibodies were applied for 1 h followed by chemiluminescence visualisation. The following primary antibodies were used: MYC (ab32072; Abcam, 1:2000 dilution) and β-actin (sc-69879, Santa-Cruz Biotechnology, 1:5000 dilution). HRP-conjugated secondary antibodies: goat anti-rabbit (sc-2301; Santa-Cruz Biotechnology 1:7500 dilution) and goat anti-mouse (A4416, Sigma-Aldrich, 1:7500 dilution).
Sodir N.M., Pellegrinet L., Kortlever R.M., Campos T., Kwon Y.W., Kim S., Garcia D., Perfetto A., Anastasiou P., Swigart L.B., Arends M.J., Littlewood T.D, & Evan G.I. (2022). Reversible Myc hypomorphism identifies a key Myc-dependency in early cancer evolution. Nature Communications, 13, 6782.
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2

Westerns on Fs288 and GDF11 in HEK293T Cells

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Westerns were conducted on samples from cells plated in a 6-well format. Briefly, 5×105 HEK293T cells were seeded in each well with growth media for 18 hours. After this time, 1.5ug of Fs288 WT or Cys56Tyr mutant vector DNA was transfected using TransIT-LT1 reagent (Mirus Bio). For GDF11, 1.5ug of WT or Arg198Gln construct DNA was transfected along with 1.5ug of Furin vector DNA to improve processing. 24hours post transfection, growth media was swapped for serum free media. Samples were collected and subject to standard western protocols. Primary antibodies against FST/Fs288 and GDF11 were purchased from R&D Biosystems (MAB66) and Abcam (ab124721), respectively. Goat anti-mouse (Millipore-Sigma; DC02L) and goat anti-rabbit (Santa Cruz; sc-2301) HRP-linked secondary antibodies, respectively, were used for detection. Western blot signal was captured using the C-DiGit blot scanner (LI-COR).
Cox T.C., Lidral A.C., McCoy J.C., Liu H., Cox L.L., Zhu Y., Anderson R.D., Uribe L.M., Anand D., Deng M., Richter C.T., Nidey N.L., Standley J.M., Blue E.E., Chong J.X., Smith J.D., Kirk E.P., Venselaar H., Krahn K.N., van Bokhoven H., Zhou H., Cornell R.A., Glass I.A., Bamshad M.J., Nickerson D.A., Murray J.C., Lachke S.A., Thompson T.B., Buckley M.F, & Roscioli T. (2019). Mutations in GDF11 and the extracellular antagonist, Follistatin, as a likely cause of Mendelian forms of orofacial clefting in humans. Human mutation, 40(10), 1813-1825.
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3

Western Blot Analysis of Rat BM-MSCs and RPE Cells

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The P0, P1, and P2 rat BM-MSCs and RPE cells were lysed with 1× cell signaling lysis buffer [26 (link)]. The cell lysates were centrifuged at 4 °C at 13800 ⨉g. The protein concentrations were determined with the Bicinchoninic Acid Protein Assay Kit (Pierce, Rockford, IL) with bovine serum albumin (BSA) as a standard. Western blot analysis was performed according to standard procedures. Proteins were resolved on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Millipore, MA), which were blocked for 2 h with 5% skim milk and incubated for 24 h with the primary polyclonal antibodies for Mertk (C-term)-Aff-Purified (AP10151PU-N; Acris antibodies, Germany) and β-actin (sc-130657; Santa Cruz Biotechnology, Santa Cruz, CA) at 4 °C, then with horseradish peroxidase-conjugated secondary antibodies (sc-2301; Santa Cruz Biotechnology), and developed with an enhanced chemiluminescent substrate or SuperSignal West Femto Maximum Sensitivity Substrate (Pierce).
Peng R.M., Hong J., Jin Y., Sun Y.Z., Sun Y.Q, & Zhang P. (2017). Mertk gene expression and photoreceptor outer segment phagocytosis by cultured rat bone marrow mesenchymal stem cells. Molecular Vision, 23, 8-19.
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4

Immunoblot Analysis of Kidney Proteins

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Immunoblot analyses were conducted with the experimenter blinded to the treatment received. The primary antibodies were rabbit anti-AQP2 (AB3274; Millipore; 1:600), sheep anti-NKCC2 (DSTT Dundee; 1:10 000), and rabbit anti-NCC (AB3553; Millipore; 1:1000); the secondary antibodies were horseradish peroxidase–conjugated goat antirabbit Ig (sc-2301; Santa-Cruz; 1:2000) and horseradish peroxidase–conjugated donkey antisheep Ig (A3415; Sigma; 1:20 000). Immunoblot analyses were conducted with the experimenter blinded to the treatment received (hence, the lack of systematic lane order). In the densitometry analysis, band density was divided by the time taken to collect the urine sample used for urinary extracellular vesicle preparation so that each result represents the abundance of antigen excreted per unit time.
Hunter R.W., Moorhouse R., Farrah T.E., MacIntyre I.M., Asai T., Gallacher P.J., Kerr D., Melville V., Czopek A., Morrison E.E., Ivy J.R., Dear J.W., Bailey M.A., Goddard J., Webb D.J, & Dhaun N. (2017). First-in-Man Demonstration of Direct Endothelin-Mediated Natriuresis and Diuresis. Hypertension (Dallas, Tex. : 1979), 70(1), 192-200.
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5

Quantitative Immunoblot Analysis of Urinary Exosomes

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Immunoblot analyses were conducted with the experimenter blinded to the treatment received. The primary antibodies were rabbit anti-AQP2 (AB3274, Millipore, 1:600), sheep anti-NKCC2 (DSTT Dundee, 1:10000) and rabbit anti-NCC (AB3553, Millipore, 1:1000); the secondary antibodies were HRP-conjugated goat anti-rabbit-Ig (sc-2301, Santa-Cruz, 1:2000) and HRP-conjugated donkey anti-sheep Ig (A3415, Sigma, 1:20000). Immunoblot analyses were conducted with the experimenter blinded to the treatment received (hence, the lack of systematic lane order). In the densitometry analysis, band density was divided by the time taken to collect the urine sample used for uEV preparation so that each result represents the abundance of antigen excreted per unit time.
Hunter R.W., Moorhouse R., Farrah T.E., MacIntyre I.M., Asai T., Gallacher P.J., Kerr D., Melville V., Czopek A., Morrison E.E., Ivy J.R., Dear J.W., Bailey M.A., Goddard J., Webb D.J, & Dhaun N. (2017). First-in-Man Demonstration of Direct Endothelin-Mediated Natriuresis and Diuresis. Hypertension (Dallas, Tex. : 1979), 70(1), 192-200.
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