Sodium borohydride

Di-tert-butyl dicarbonate, sodium borohydride, p-toluene sulphonic acid and anhydrous methanol were procured from SD Fine Chemicals (Mumbai, India). Polyphosphoric acid and anhydrous methanol were purchased from Loba Chemicals (New Delhi, India). p-Iodoaniline, diethylnitrosamine (DEN) and 2,4-dinitrophenylhydrazine were procured from Sigma-Aldrich (Bengaluru, India). ALT and AST kit was acquired from the Transasia Bio-Medicals Pvt Ltd (Baddi, India). All other chemicals were obtained from Himedia (Mumbai, India). All the solvents and chemicals were of analytical grades with 99% purity and in house distilled water was used throughout the experiment.

Hydrogen tetrachloroaurate(iii) trihydrate (HAuCl₄·3H₂O, LOBA chemicals, 99.999%). Polyvinylpyrrolidone (PVP, 30 K, 99%), 7,12-dimethylbenz[a]anthracene (DMBA, 99%), and dimethyl sulfoxide (DMSO, D2560, 99%) were purchased from Sigma-Aldrich. Sodium borohydride (NaBH₄, sd-fine chemicals 98%), N-cetyl N,N,N,-trimethyl ammonium bromide (CTAB, Merck, 99%), silver nitrate (AgNO₃, Sigma Aldrich, 99%), l-ascorbic acid (vitamin C, 99%), hydrogen peroxide (H₂O₂, 50%) and xylene were purchased from El-Nasr. phosphate buffer saline (PBS) was purchased from GeneTech, purified water with a resistivity of 18 MW from the Millipore MilliQ water purification system.

Acetone, ethylene glycol, silver nitrate, ammonium solution, potassium chloride, polyvinylpyrrolidone (K 90), dichloromethane, sodium chloride, and sodium borohydride were purchased from SD Fine Chemicals Ltd., Mumbai, India. All the chemicals of analytical grade were used without any further purification. The deionized water (18 MΩ) used in the experiments was obtained from Elga Pure lab Q7/15 water purifier Woodridge, IL, USA.