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Goat anti rabbit secondary antibodies conjugated to horseradish peroxidase

Manufactured by Santa Cruz Biotechnology
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Goat anti-rabbit secondary antibodies conjugated to horseradish peroxidase are a type of immunological reagent used in various laboratory techniques. These antibodies are designed to bind to and detect rabbit primary antibodies, with the horseradish peroxidase enzyme serving as a label for visualization or quantification purposes.

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Lab products found in correlation

Quick start bradford protein assay kit, by Bio-Rad (1 mentions) Anti enos, by Cell Signaling Technology (1 mentions) Anti phospho enos thr495, by Cell Signaling Technology (1 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Anti phospho enos ser1177, by Cell Signaling Technology (1 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions) Sds page, by Bio-Rad (1 mentions) Image lab 6, by Bio-Rad (1 mentions) Enhanced chemiluminescence detection assay, by Bio-Rad (1 mentions) Semi dry blotter, by Bio-Rad (1 mentions)

Spelling variants of this product

Horseradish peroxidase (230 citations) Secondary antibodies conjugated to horseradish peroxidase (17 citations) Anti-rabbit antibodies (6 citations) Secondary antibodies conjugated to peroxidase (4 citations) Anti-goat secondary antibodies (3 citations) Goat anti-rabbit antibodies (3 citations) Horseradish peroxidase-conjugated rabbit anti-goat (2 citations) Horseradish peroxidase anti-rabbit (2 citations) Goat antibodies (2 citations) Rabbit anti-TM (2 citations)

3 protocols using goat anti rabbit secondary antibodies conjugated to horseradish peroxidase

1

Quantifying Soluble Proteins in Wheat and Rice

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Total soluble proteins in flag leaves and seeds were extracted from each T2 transgenic wheat event, one non-transgenic wheat variety (BW), and one rice variety (Nipponbare) at 20 d after anthesis. Proteins were extracted by grinding and homogenizing samples in protein extraction buffer (Fu et al.2008 (link)). Protein concentrations were estimated by using Quick Start® Bradford Protein Assay kit (Bio Rad® Inc., Hercules, CA) according to manufacturer instructions. Samples were diluted in the buffer to maintain equal concentration for equal loading. Equal loading was also maintained by checking stained gels. Protein separation was performed by loading 40 μg of each sample on 10% (w/v) sodium dodecyl sulfate (SDS) polyacrylamide gels. SDS-PAGE separated proteins were then transferred to polyvinylidene difluoride (PDVF) membranes for immunoblotting. PDVF membranes with the transferred proteins were probed for rice SSI protein using rabbit polyclonal anti-SSI antibodies (AnaSpec, Inc., Fremont, CA), and goat anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Inc., Santa Cruz, CA).
Tian B., Talukder S.K., Fu J., Fritz A.K, & Trick H.N. (2018). Expression of a rice soluble starch synthase gene in transgenic wheat improves the grain yield under heat stress conditions. In Vitro Cellular & Developmental Biology, 54(3), 216-227.
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2

Cerebral Endothelial Cell Signaling

Cited 1 time
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Cerebral blood vessels and brain microvascular endothelial cells samples were lysed in RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.5% deoxycholic acid, 0.1% SDS, 1 mM EDTA pH 8.0, 1% IGEPAL CA-630) and equal volumes were mixed with SDS sample buffer, boiled, and analyzed on 4–12% SDS polyacrylamide gels. Proteins were transferred to PVDF membranes (Millipore), blocked with 5% milk in TBS/0.1% Tween-20 (TBST) and incubated with polyclonal anti-phospho-eNOS (Ser1177), anti-phospho-eNOS (Thr495) and anti-eNOS (1:1,000, Cell Signaling cat. #9571, 9574 and 9572, respectively). Membranes were washed in TBST, incubated with goat anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Santa Cruz Biotechnology), and protein bands were visualized with Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) or Clarity Western ECL Substrate (Bio-Rad) on a Bio-Rad ChemiDoc MP Imaging System33 (link).
Faraco G., Brea D., Garcia-Bonilla L., Wang G., Racchumi G., Chang H., Buendia I., Santisteban M.M., Segarra S.G., Koizumi K., Sugiyama Y., Murphy M., Voss H., Anrather J, & Iadecola C. (2018). Dietary salt promotes neurovascular and cognitive dysfunction through a gut-initiated TH17 response. Nature neuroscience, 21(2), 240-249.
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3

Quantifying Notch Protein Expression

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Cell lysates (50μg) were boiled in the presence of SDS sample buffer (NuPAGE LDS sample buffer; Invitrogen) for 10 minutes and subjected to electrophoresis on 10% SDS-PAGE (Bio-Rad Laboratories). Proteins were transferred to PVDF membranes using a semidry blotter (Bio-Rad) and incubated in blocking solution (5% nonfat dry milk in TBS containing 0.1% Tween-20) for 1 hour to reduce nonspecific binding. Immunoblots were performed using anti-GAPDH (1:1000), NOTCH1-3 (1:1000), and NOTCH4 (1:500), antibodies followed by goat anti-rabbit secondary antibodies, conjugated to horseradish peroxidase (1:2000) in 5% milk (Santa Cruz Biotechnology). Western blots were developed using an enhanced chemiluminescence detection assay following the manufacturer's directions (Bio-Rad). Protein bands were quantified using Image Lab 6.0.1 (Bio-Rad).
Sabol H.M., Amorim T., Ashby C., Halladay D., Anderson J., Cregor M., Sweet M., Nookaew I., Kurihara N., Roodman G.D., Bellido T, & Delgado-Calle J. (2022). Notch3 signaling between myeloma cells and osteocytes in the tumor niche promotes tumor growth and bone destruction. Neoplasia (New York, N.Y.), 28, 100785.
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