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A1r si laser scanning confocal spectral microscope

Manufactured by Nikon
Sourced in Japan

The A1R-Si laser scanning confocal spectral microscope is designed for high-resolution, multi-channel imaging and spectral analysis. It features a spectral detector that enables the simultaneous capture of multiple fluorescence signals across a wide wavelength range. The microscope system employs laser excitation and high-speed scanning mirrors to provide optical sectioning capabilities for 3D imaging of samples.

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2 protocols using a1r si laser scanning confocal spectral microscope

1

Visualizing HMF Cell Nuclei

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HMF cells cultured in glass-bottom dishes were fixed with ice-cold 100% methanol for 15 min. Subsequently, the cells were incubated with the cell-permeable nucleic acid stain Hoechst 33342 (20μg/ml) for 30 min. The stained nuclei of HMF cells were imaged using the Nikon A1R-Si laser scanning confocal spectral microscope.
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2

Nile Red Staining for Lipid Quantification

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Nile Red staining was performed as described by Jendrossek et al. (2007 (link)) with modifications by Aneesh et al. (2016 (link)). The cells were imaged on a Nikon A1R-Si laser scanning confocal spectral microscope with 50× magnification (Nikon Corporation, Tokyo, Japan) excited at 561 nm.
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