Mouse anti nqo 1

Goat polyclonal anti-α SMA, mouse monoclonal anti-E-ca, mouse monoclonal anti Nrf2, mouse monoclonal anti HO-1, mouse anti-NQO1, mouse anti-Keap1, and mouse anti β-actin were purchased from Santa Cruz Biotechnology (San Francisco, CA, USA), while Smad2, Smad3, p-Smad2, p-Smad3 were bought from Cell Signaling Technology (3 Trask Lane Danvers, Danvers, MA, USA). Western blot analysis was performed as previously described [61 (link)]. Briefly, equal amounts of protein extracts were loaded to SDS-PAGE 10% gels, and the proteins were transferred to PVDF membranes (Millipore, Temecula, CA, USA). Then the blots were incubated with primary antibodies after blocking with 5% fat-free milk and washed with TBST. Next, the membranes were then incubated with HRP-conjugated secondary antibodies and washed repeated with TBST. After then, the membranes were added with enhanced chemiluminescence kit (Walterson Biotechnology Inc., Beijing, China) and imaged with G BOX EF Chemi HR16 gel imaging system (Syngene, Frederick, MD, USA), Finally, the bands of blots were analyzed and quantified with ChemiDoc™ XRS system (Bio-Rad, Hercules, CA, USA) with Quantity One software version 4.6 (Bio-Rad Laboratories, Inc., Bio-Rad, Hercules, CA, USA). The blots were repeated at least three times for each condition.