Ift88fl fl

All experimental procedures were performed in compliance with animal protocols approved by the IACUC at Boston Children’s Hospital and Washington University in St. Louis. Mice aged 3–5 weeks at the start of experiments were used throughout. Male and female mice were used in this study at ratios dependent on litters available and with equal distributions across experiments conducted extemporaneously. C57BL6/J, Vglut2-ires-Cre (028863; Jackson Labs), Vgat-IRES-Cre (016962; Jackson Labs), IFT88fl/fl (022409; Jackson Labs) and IGF1Rfl/fl (mouse lines were obtained from Jackson Laboratories. The connexin 57cre (Cx57-Cre) mouse line was described in (Hirano et al., 2016 ).

All the animals in the study were handled according to protocols approved by the University of Texas (UT) Southwestern Institutional Animal Care and Use Committee, and the mouse colonies were maintained in a barrier facility at UT Southwestern, in agreement with the State of Texas legal and ethical standards of animal care. The newly generated Gpr161 floxed mouse strain was maintained in a C57BL/6 background (Hwang et al., 2017 ). Nestin-Cre mice (B6.Cg-Tg(Nes-cre)1Kln/J, stock no. 003771), Atoh1-Cre mice (B6.Cg-Tg(Atoh1-cre)1Bfri/J, stock no. 011104), hGFAP-Cre mice (FVB-Tg(GFAP-cre) 25Mes/J, stock no. 004600), SmoM2 mice (Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}/J, stock no. 005130), and Ift88^fl/fl (B6.129P2-Ift88^tm1Bky/J, stock no. 022409) mice were obtained from Jackson Laboratory (Bar Harbor, ME). Both male and female mice were analyzed in all experiments. We did not notice any difference between wild-type or heterozygous animals for both Gpr161 and Ift88 floxed alleles with or without Cre recombinase, and, thus, all were included as littermate controls, as mentioned in the respective figure legends. Noon of the day on which a vaginal plug was found was considered E0.5. Tail DNAs were used for genotyping embryos. Genotyping details are provided in the Supplemental Experimental Procedures.