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Eif4h

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EIF4H is a protein involved in the regulation of translation initiation. It is a member of the eIF4 complex, which plays a crucial role in the recruitment of ribosomes to the mRNA. EIF4H functions to enhance the helicase activity of the eIF4A protein, facilitating the unwinding of secondary structures in the mRNA, which is necessary for efficient translation.

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Lab products found in correlation

Plcγ1, by Cell Signaling Technology (1 mentions) α parvin, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Goat anti mouse igg hrp, by Abmart (1 mentions) Cyclin d1, by Abcam (1 mentions) C myc, by Abcam (1 mentions) β actin, by Proteintech (1 mentions) Rbm10, by Merck Group (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Puromycin, by Merck Group (1 mentions) Eif4g, by Cell Signaling Technology (1 mentions) Eif4e, by Cell Signaling Technology (1 mentions) P eif4e, by Cell Signaling Technology (1 mentions) P eif4b, by Cell Signaling Technology (1 mentions) Eif4a, by Cell Signaling Technology (1 mentions) P eif4g, by Cell Signaling Technology (1 mentions) Eif4b, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Rabbit anti-eIF4H (2 citations)

3 protocols using eif4h

1

Quantifying Cell Signaling Proteins

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PLC-γ1, eIF4H, α-Parvin and ß-actin were obtained from Cell Signaling Technology (Danvers, MA, USA).
Ferreira E., Shaw D.M, & Oddo S. (2016). Identification of learning-induced changes in protein networks in the hippocampi of a mouse model of Alzheimer's disease. Translational Psychiatry, 6(7), e849-.
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2

Protein Expression Analysis by Western Blot

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Protein levels were detected by Western blot analysis as previously described(38). The following primary antibodies were used: RBM10 (cat No. HPA034972, 1:10,000, Sigma), KRAS (cat No.sc-30,1:200, Santa Cruz Biotechnologies Inc.), EGFR (cat No.D38B1,1:1000,Cell signalling Technology), β-actin (cat No.66009–1-Ig,1:10,000,Proteintech), cleaved caspase 3 (cat No. 9661, Asp175, 1:500, Cell signalling Technology), cleaved PARP (cat No.D64E10,Asp214,1:500, Cell signalling Technology), EIF4H (cat No. 3469,D85F2,1:1000, Cell signalling Technology), c-Myc (cat No.ab32072,1:5000,abcam), cyclin D1 (cat No.ab134175,1:1000,abcam). The following secondary antibodies were used: Goat anti-rabbit IgG HRP (cat. No. 458, 1:5000, MBL), Goat anti-mouse IgG HRP (cat No.M21001,1:5000, Abmart).
Zhang S., Bao Y., Shen X., Pan Y., Sun Y., Xiao M., Chen K., Wei H., Zuo J., Saffen D., Zong W.X., Sun Y., Wang Z, & Wang Y. (2020). RNA binding motif protein 10 suppresses lung cancer progression by controlling alternative splicing of eukaryotic translation initiation factor 4H. EBioMedicine, 61, 103067.
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3

Western Blotting Protein Analysis

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Western blotting analyses were performed as described previously (Marabita et al., 2016 (link)). Antibodies for P-Akt, Akt, eIF4E, P-eIF4E, P-eIF4B, eIF4B, eIF4A, eIF4H, eIF4G, P-eIF4G, 4E-BP1, P-S6, S6 were taken from Cell Signaling. Actin was from Santa Cruz, and puromycin from Millipore. All quantifications of the western blots were done on at least four different blots for each protein, in each condition. Differences between groups were assessed using Student's t-test. Significance was defined as a value of P < 0.05 (95% confidence).
Pereira M.G., Dyar K.A., Nogara L., Solagna F., Marabita M., Baraldo M., Chemello F., Germinario E., Romanello V., Nolte H, & Blaauw B. (2017). Comparative Analysis of Muscle Hypertrophy Models Reveals Divergent Gene Transcription Profiles and Points to Translational Regulation of Muscle Growth through Increased mTOR Signaling. Frontiers in Physiology, 8, 968.
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