Caffeic acid

Adenosine 5’-diphosphate (ADP), thrombin-6 receptor activating peptide (TRAP-6), and collagen were purchased from Sigma-Aldrich (St. Louis, Missouri/MO, U.S.A). Phorbol 12-myristate 13-acetate (PMA) was obtained from Tocris-Cookson. Antimycin A was obtained from Genetica y Tecnologia spa (GENYTEC, Chile). Standards for caffeic acid, myricetin, rutin, quercetin, kaempferol, kuromanin chloride (cyanidin 3-O-glucoside), and myrtillin (delphinidin 3-O-glucoside) were from Cayman Chemical (Ann Arbor, MI). All used solvents were of HPLC quality and purchased from Burdick and Jackson (Muskegon, MI, USA).

H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) was purchased from Life Technologies (Carlsbad, CA, USA). Zileuton (N-[1-benzo(b)thien-2-ylethyl]-N-hydroxy-urea, CAS 111406-87-2) was purchased from Sigma-Aldrich (St. Louis, MO, USA) and NDGA (4,4′-(2,3-dimethyl-1,4-butanediyl)bis-1,2-benzenediol, CAS 500-38-9) was purchased from Cayman Chemical (Ann Arbor, MI, USA).
Human recombinant 5-LO lysate, 13(S)-HpODE (13S-hydroperoxy-9Z,11E-octadecadienoic acid, CAS 33964-75-9), YS121 (2-[[4-chloro-6-[(2,3-dimethylphenyl)amino]-2-pyrimidinyl]thio]-octanoic acid, CAS 916482-17-2), caffeic acid (3,4-dihydroxy cinnamic acid, CAS 331-39-5), CDC (cinnamyl 3,4-dihydroxy-alpha-cyanocinnamate, CAS 132465-11-3), CAY10606 (2-[(3-chlorophenyl)methyl]-5-hydroxy-1H-benz[g]indole-3-carboxylic acid, ethyl ester, CAS 1159576-98-3), and CAY10649 ((Z)-2-(4-chlorophenyl)-5-(4-methoxybenzylidene)thiazol-4(5H)-one, CAS 1272519-89-7) were from Cayman Chemical. DMSO (dimethyl sulfoxide) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
PF4191834 was kindly donated by Qurient, Inc. (Seongnam-si, South Korea).
Fluorescence and absorbance measurements were performed using the SpectraMax M5 microtiter plate reader (Molecular Devices, Sunnyvale, CA, USA).

Phenolic standards: 2,5-DHBA, 3,4-DHBA, apigenin, caffeic acid, chrysin, kaempferol, luteolin, myricetin, naringenin, p-coumaric acid and quercetin were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). Ethanol (LC-MS purity) was purchased from Sigma-Aldrich, Merck (St. Louis, MO, USA). Methanol (LC-MS purity) and acetonitrile (ACN; LC-MS purity) were purchased from VWR Chemicals BDH® (Radnor, PA, USA). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)) were purchased from Alfa Aesar (Haverhill, MA, USA). The (±)-6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox) was purchased from Sigma-Aldrich, Merck. Aluminium chloride 6-hydrate was purchased from Gram-Mol (Zagreb, Croatia).
Milli-Q water was obtained using connected Ultrapure Water Systems (GenPure UV-TOC/UF xCAD plus) and Milli-Q water purification system (<0.055 μS/cm, Milli-Q Model Pacific TII 12; Thermo Fisher Scientific, Waltham, MA, USA).