Spermidine trihydrochloride

Spermidine trihydrochloride (SPD) was purchased from Nacalai Tesque (Kyoto Japan). Polyethylene Glycol 6,000 (PEG), Dextran 200,000 (DEX) and the antioxidant 2-mercaptoethanol (2-ME) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). T4 GT7 bacteriophage DNA (166 kbp with a contour length of 57 μm) and Tris-hydrochloride acid buffer (ph7.5) were purchased from Nippon Gene (Tokyo, Japan). The dimeric cyanine fluorescent dye YOYO-1 (1,10-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-[(3-methylbenzo-1,3-oxazol-2-yl)methylidene]-l,4-dihydroquinolinium] tetraiodide) was obtained from Molecular Probes Inc. (OR, USA).

Agmatine and cadaverine were purchased from Tokyo Chemical Industry (Tokyo, Japan). N-carbamoylputrescine was synthesized by Life Chemical (Kiev, Ukraine). The chemicals 1,4-butanediammonium dichloride and L(+)-arginine hydrochloride were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). Spermidine trihydrochloride and spermine tetrahydrochloride were purchased from Nacalai Tesque (Kyoto, Japan). All other reagents were of analytical grade.

Spermidine trihydrochloride (SPD) was purchased from Nacalai Tesque (Kyoto, Japan). Bis(3-aminopropyl)amine (NSPD) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The fluorescent cyanine dye YOYO-1 (1,10-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-[(3-methylbenzo-1,3-oxazol-2-yl)methylidene]-l,4-dihydroquinolinium] tetraiodide) was purchased from Molecular Probes, Inc. (Eugene, OR, USA). The antioxidant 2-mercaptoethanol (2-ME) and calf thymus DNA (CT DNA: 8–15 kbp) were purchased from Wako Pure Chemical Industries (Osaka, Japan). T4 GT7 phage DNA (contour length: 57 μm, 166 kbp) was purchased from Nippon Gene (Toyama, Japan). Plasmid DNA (Luciferase T7 Control DNA, 4331 bp) containing a T7 RNA polymerase promotor sequence was purchased from Promega (Madison, WI, USA). Other chemicals obtained from commercial sources were of analytical grade.