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Anti detyrosinated α tubulin

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Anti-detyrosinated α-tubulin is a primary antibody that specifically recognizes the detyrosinated form of α-tubulin. Detyrosination is a post-translational modification that occurs on α-tubulin, and this antibody can be used to detect and study this modified form of the protein.

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Lab products found in correlation

Anti acetylated α tubulin, by Merck Group (2 mentions) Hoechst dye, by Thermo Fisher Scientific (2 mentions) Acti stain 488, by Cytoskeleton (2 mentions) Acti stain 555, by Cytoskeleton (2 mentions) Anti α tubulin, by Merck Group (2 mentions) Anti acetyl α tubulin lys40 d20g3 xp rabbit mab, by Cell Signaling Technology (2 mentions) Monoclonal anti α tubulin clone dm1a, by Merck Group (2 mentions) Ac 15, by Merck Group (2 mentions) Anti α actinin 4, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by SCBT (1 mentions) Anti ha, by Fortis Life Sciences (1 mentions) Anti actin, by Merck Group (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti rhoa, by Cytoskeleton (1 mentions) Anti rhoa, by Merck Group (1 mentions) Anti myc, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Hoescht 33342, by Merck Group (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Fv1000 confocal laser scanning microscope, by Olympus (1 mentions)

Close spelling variants of this product

α-tubulin (275 citations) Anti-α-tubulin (184 citations) Anti-tubulin (130 citations) Anti-detyrosinated tubulin (4 citations) Detyrosinated tubulin (3 citations) Detyrosinated α-tubulin (2 citations)

5 protocols using anti detyrosinated α tubulin

1

Immunolabeling of Cytoskeletal Proteins

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The following primary antibodies were used: anti-α-actinin 4 (mouse, sc-393495; Santa Cruz Biotechnology [SCBT]), anti-α-actinin 1 (rabbit, ab68194; Abcam), anti-α-actinin 4 (mouse, sc-390205; SCBT), anti-α-tubulin (mouse, T5168; Sigma), anti-α-tubulin (rat, sc-53029; SCBT), anti-acetylated α-tubulin (mouse, T6793; Sigma), anti-detyrosinated α-tubulin (rabbit, 48389; Abcam), ActiStain-488 (PHDG1; Cytoskeleton), ActiStain-555 (PHDH1-A; Cytoskeleton), anti-heat shock protein 70 (HSP70) (chicken, SPC-178D; StressMarq), anti-IncA (rabbit; T. Hackstadt), and anti-MOMP (goat, 1621; ViroStat). The following secondary reagents were used: Hoechst dye (H1399), and goat and donkey anti-mouse, anti-goat, and anti-rabbit Alexa Fluor 488-, 555-, or 647-conjugated secondary antibodies (Invitrogen, Jackson ImmunoResearch). Donkey anti-chicken (IgY), anti-mouse, or anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Invitrogen.
Haines A., Wesolowski J, & Paumet F. (2023). Chlamydia trachomatis Subverts Alpha-Actinins To Stabilize Its Inclusion. Microbiology Spectrum, 11(1), e02614-22.
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2

Comprehensive Antibody Panel for Cell Analysis

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The following primary antibodies were used: anti-HA (chicken, A190-106A; Bethyl Laboratories), anti-actin (rabbit, A2066; Sigma), anti-myc (mouse, 9E10 clone; Juan Bonifacino, NIH), anti-myc (rabbit, 2278; Cell Signaling), anti-RhoA (mouse, ARH04; Cytoskeleton), anti-RhoA (mouse, SAB1400017; Sigma), anti-FLAG (mouse, F1804; Sigma), anti-FLAG (rabbit, 600-401-383; Rockland Immunochemical), anti-ARF1 (mouse, sc-53168; SCBT), anti-α-tubulin (mouse, T5168; Sigma), anti-acetylated α-tubulin (mouse, T6793; Sigma), anti-detyrosinated α-tubulin (rabbit, 48389; Abcam), ActiStain-488 (PHDG1; Cytoskeleton), Actistain-555 (PHDH1-A; Cytoskeleton), anti-heat shock protein 70 (HSP70) (chicken, SPC-178D; StressMarq), anti-InaC (mouse; T. Hackstadt), anti-IncA (rabbit; T. Hackstadt), and anti-MOMP (goat, 1621; ViroStat). The following secondary reagents were used: Hoechst dye (H1399) and goat and donkey anti-mouse, anti-goat, anti-rabbit, and anti-chicken (IgY) IgG Alexa Fluor 488-, 555-, or 647-conjugated secondary antibodies (Invitrogen). Donkey anti-chicken, anti-mouse, or anti-rabbit IgG and IgY horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Invitrogen.
Haines A., Wesolowski J., Ryan N.M., Monteiro-Brás T, & Paumet F. (2021). Cross Talk between ARF1 and RhoA Coordinates the Formation of Cytoskeletal Scaffolds during Chlamydia Infection. mBio, 12(6), e02397-21.
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3

Immunofluorescence Staining for Acetylated and Detyrosinated Tubulin

Cited 1 time
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Cells were grown on glass coverslips and then fixed in 100% cold methanol (10 min, 4 °C). Immunofluorescence staining was performed as previously described [10 (link)] with the following antibodies: Anti-acetyl-α-tubulin (Lys40) (D20G3) XP Rabbit mAb (1:1000; Cell Signaling), Anti-detyrosinated-α-tubulin (1:1000; Abcam), Monoclonal Anti-detyrosinated- α-tubulin (1:10,000: RevMAb), Monoclonal Anti-α-tubulin Clone DM1A (1:5000; Sigma Aldrich) and β-actin (1:2000; AC-15, Sigma). Anti-IgG antibodies conjugated to Alexa-488 (1:1000; Molecular Probes) were used for secondary detection and Hoescht 33342 (1:5000; Sigma) was used for nuclear staining. Images were acquired using an Olympus FV1000 laser scanning confocal microscope (Olympus, Center Valley, PA). The deTyr-Tubulin antibody (RevMab) was developed by Takashi Hotta and Ryoma Ohi [23 (link)] and is now commercially available through RevMab under catalogue number RM444 (https://www.revmab.com/index.php/product/anti-detyrosinated-alpha-tubulin-rabbit-monoclonal-antibody-clone-rm444/).
Thompson K.N., Ju J.A., Ory E.C., Pratt S.J., Lee R.M., Mathias T.J., Chang K.T., Lee C.J., Goloubeva O.G., Bailey P.C., Chakrabarti K.R., Jewell C.M., Vitolo M.I, & Martin S.S. (2022). Microtubule disruption reduces metastasis more effectively than primary tumor growth. Breast Cancer Research : BCR, 24, 13.
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4

Immunoblot Analysis of Apoptosis Markers

Cited 1 time
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Subconfluent MDA-MB-231 TD, MDA-MB-436 TD, BT-549 TD cells were treated with either Vinorelbine, vehicle control, or Staurosporine. All cells, any suspended and attached, were harvested, lysed with a RIPA lysis buffer, and analyzed by immunoblot as previously described [24 (link)] with the following antibodies: Anti-Poly (ADP-ribose) polymerase (PARP; 1:1000, SCBT), Anti-acetyl-α-tubulin (Lys40) (D20G3) XP Rabbit mAb (1:1000; Cell Signaling), Anti-detyrosinated- α-tubulin Monoclonal (1:10,000: RevMAb) or Anti-detyrosinated-α-tubulin (1:1000; Abcam), Monoclonal Anti-α-tubulin Clone DM1A (1:2000; Sigma Aldrich) and β-actin (1:5000; AC-15, Sigma).
Thompson K.N., Ju J.A., Ory E.C., Pratt S.J., Lee R.M., Mathias T.J., Chang K.T., Lee C.J., Goloubeva O.G., Bailey P.C., Chakrabarti K.R., Jewell C.M., Vitolo M.I, & Martin S.S. (2022). Microtubule disruption reduces metastasis more effectively than primary tumor growth. Breast Cancer Research : BCR, 24, 13.
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5

Profiling Microtubule Modifications in Heart Tissue

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Protein lysates from heart tissues were loaded on and separated by a 12% SDS-PAGE and then transferred to PVDF membranes (EMD Millipore, MA, USA). The membranes were probed overnight with primary antibodies at 4°C. The primary antibodies were anti-detyrosinated α-tubulin (Abcam, Cambridge, CB, UK), anti-acetylated α-tubulin (Abcam, Cambridge, CB, UK), anti-α-tubulin (Abcam, Cambridge, CB, UK), anti-tubulin tyrosine ligase (Proteintech, Rosemont, IL, USA), anti-HDAC6 (Abcam, Cambridge, CB, UK), and anti-GAPDH (Abcam, Cambridge, CB, UK). Membranes were incubated with appropriate secondary antibodies for 2 h at room temperature. Membranes were imaged using Odyssey infrared fluorescence imager (LI-COR, Lincoln, NE, USA). Some of the signals were detected using Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, MA, USA).
Chen H.H., Zhao P., Tian J., Guo W., Xu M., Zhang C, & Lu R. (2017). The Effects of Guizhi Gancao Decoction on Pressure Overload-Induced Heart Failure and Posttranslational Modifications of Tubulin in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 2915247.
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