Anti cdc25b

Manufactured by Santa Cruz Biotechnology

Anti-Cdc25B is a laboratory reagent that targets the Cdc25B protein. Cdc25B is a phosphatase enzyme involved in cell cycle regulation. This product can be used to study the role of Cdc25B in biological processes.

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Lab products found in correlation

2 protocols using anti cdc25b

Western Blot Analysis of Cell Cycle Proteins

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Cells were lysed by sonication in RIPA buffer and the protein concentration was determined using a Nanodrop. Equal protein amounts were resolved by SDS-PAGE (10%). Proteins were transferred to PVDF membranes (Bio-Rad) by electroblotting. The membranes were blocked with 5% nonfat dry milk in TBST buffer and analyzed by Western analysis with anti-Flag (Sigma), anti-Cdc25A (Thermo Scientific), and/or anti-β-actin (Sigma). Anti-Cdc25B, anti-Cdc25C, anti-cyclin A2, anti-cyclin E1, anti-cyclin D1, anti-cyclin D3, and anti-pRB were purchased from Santa Cruz Biotechnology.

Lozada E.M., Andrysik Z., Yin M., Redilla N., Rice K, & Stambrook P.J. (2016). Acetylation and deacetylation of Cdc25A constitutes a novel mechanism for modulating Cdc25A functions with implications for cancer. Oncotarget, 7(15), 20425-20439.

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Western Blotting Analysis of Cell Signaling

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Western blotting assay was performed as described previously (Cao et al, 2011; Zhou et al, 2009). The following antibodies were used in this study: anti-FOXM1 (Santa Cruz, sc-500); anti-acetylated lysine (Cell Signaling Technology, #9681); anti-CDK1 (Abcam, ab133327); anti-MPM-2 (Millipore, 05-368); anti-p300 (Santa Cruz, sc-584); anti-Plk1 (Santa Cruz, sc-17783); anti-CDC25B (Santa Cruz, sc-326); anti-CyclinB1 (Santa Cruz, sc-245); anti-Aurora B (Cell Signaling Technology, 3049S); anti-FLAG (Sigma); anti-Survivin (Cell Signaling Technology, 2808); anti-HA-tag (Cell Signaling Technology); anti-GAPDH (Santa Cruz, sc-47724).

Lv C., Zhao G., Sun X., Wang P., Xie N., Luo J, & Tong T. (2016). Acetylation of FOXM1 is essential for its transactivation and tumor growth stimulation. Oncotarget, 7(37), 60366-60382.

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