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Aperio versa digital pathology scanner

Manufactured by Leica
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Sourced in Japan

The Aperio VERSA Digital Pathology Scanner is a high-performance slide scanning system designed for digital pathology applications. It captures high-resolution digital images of microscope slides for analysis and review. The scanner utilizes advanced optical and imaging technology to provide accurate and reliable digital slide capture.

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Lab products found in correlation

Antibody for cd14, by Cell Signaling Technology (2 mentions) Biosystems refine detection kit, by Leica (2 mentions) Bz x700, by Keyence (2 mentions) Fluorescently labeled primary antibodies, by Cell Signaling Technology (2 mentions) Andaxio scan z1, by Leica (2 mentions) D8h1x, by Cell Signaling Technology (2 mentions) Bond rx, by Leica (1 mentions) Bond rx autostainer, by Leica (1 mentions) Tpl s eslide manger database, by Leica (1 mentions) Tcs sp5, by Leica (1 mentions) Imagescope software, by Leica (1 mentions) Imaris software, by Oxford Instruments (1 mentions) Dm6000b microscope, by Leica (1 mentions)

Close spelling variants of this product

Aperio Versa (29 citations) Aperio Digital Pathology (11 citations) Aperio Versa Scanner (8 citations) Digital pathology scanner (4 citations) Aperio scanners (2 citations)

7 protocols using aperio versa digital pathology scanner

1

Multiplexed Immunofluorescence Analysis of SARS-CoV-2

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Formalin fixed and paraffin embedded lung parenchymal samples were stained for SARS-CoV-2 nucleocapsid (N), ASC, and CD14 and IF was analyzed on the Leica Bond RX automated staining platform using the Leica Biosystems Refine Detection Kit (Leica). The antibody for SARS Nucleocapsid (Novus) was run with citrate antigen retrieval and tagged with Alexa Fluor 488 Tyramide (Life). Following citrate stripping, the antibody for CD14 (Cell Signaling) was incubated and tagged with Alexa Fluor 594 Tyramide (Life). Following EDTA stripping, staining for ASC (Santa Cruz) was analyzed using antibody tagged with Alexa Fluor 647 Tyramide (Life). Samples were counterstained with DAPI. Slides were scanned using an Aperio Versa Digital Pathology Scanner (Leica) and analyzed with Aperio ImageScope v12.4.3 software (Leica). Slides were also analyzed by confocal microscopy as described above.
Junqueira C., Crespo Â., Ranjbar S., Lewandrowski M., Ingber J., de Lacerda L.B., Parry B., Ravid S., Clark S., Ho F., Vora S.M., Leger V., Beakes C., Margolin J., Russell N., Kays K., Gehrke L., Adhikari U.D., Henderson L., Janssen E., Kwon D., Sander C., Abraham J., Filbin M., Goldberg M.B., Wu H., Mehta G., Bell S., Goldfeld A.E, & Lieberman J. (2021). SARS-CoV-2 infects blood monocytes to activate NLRP3 and AIM2 inflammasomes, pyroptosis and cytokine release. Research Square.
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2

Multiplex Immunofluorescence Imaging of SARS-CoV-2 in Lung Tissue

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Formalin-fixed and paraffin-embedded lung parenchymal samples were stained for SARS-CoV-2 N, ASC and CD14, and immunofluorescence was analysed on the Leica Bond RX automated staining platform using the Leica Biosystems Refine Detection Kit (Leica). The antibody for SARS nucleocapsid (Novus) was run with citrate antigen retrieval and tagged with Alexa Fluor 488 Tyramide (Life). After citrate stripping, the antibody for CD14 (Cell Signaling) was incubated and tagged with Alexa Fluor 594 Tyramide (Life). After EDTA stripping, staining for ASC (Santa Cruz) was analysed using antibodies tagged with Alexa Fluor 647 Tyramide (Life). EDTA stripping was performed before anti-CD31 or anti-E-cadherin staining tagged to Alexa Fluor 555 Tyramide (Life). The samples were counterstained with DAPI. The slides were scanned using the Aperio Versa Digital Pathology Scanner (Leica) and analysed using Aperio ImageScope v.12.4.3 (Leica). The slides were also analysed by confocal microscopy as described above.
Junqueira C., Crespo Â., Ranjbar S., de Lacerda L.B., Lewandrowski M., Ingber J., Parry B., Ravid S., Clark S., Schrimpf M.R., Ho F., Beakes C., Margolin J., Russell N., Kays K., Boucau J., Adhikari U.D., Vora S.M., Leger V., Gehrke L., Henderson L.A., Janssen E., Kwon D., Sander C., Abraham J., Goldberg M.B., Wu H., Mehta G., Bell S., Goldfeld A.E., Filbin M.R, & Lieberman J. (2022). FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation. Nature, 606(7914), 576-584.
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3

Multimodal Imaging of HCC Samples

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A collection of fresh frozen hepatocellular carcinoma (HCC, n=38) samples was used in the
present study. HCC specimens were collected at the University of California, San Francisco (San Francisco, CA). Institutional
Review Board approval was obtained and informed consent was obtained from all subjects. See Gordan et al., manuscript
in preparation, for further details of this sample set. Immunofluorescence staining and imaging was performed at
the Gladstone Institutes Histology and Light Microscopy core using fluorescently-labeled primary antibodies (all from Cell
Signaling, Danvers, MA) to CTNNB1 (Mouse mAb L54E2, conjugated to Alexa Fluor® 555, 1:200, Cat#5612) YAP
(Rabbit mAb D8H1X, conjugated to Alexa Fluor® 488, 1:200, Cat#14729) and LKB1 (Rabbit mAb, 1:250,
Cat#13031, followed by goat anti-rabbit conjugated to Alexa Fluor® 633, 1:200). Staining and imaging for
DAPI/YAP/LKB1 was first performed on 38 samples, and 5 months later serial sections for 25 of these samples were
stained/imaged for DAPI/YAP/CTNNB1. Stitched images of entire sections were acquired at 20× (0.32μm per pixel)
on automated slide scanners (BZ-X700; Keyence, Osaka, Japan; Aperio VERSA Digital Pathology Scanner, Leica Biosystems, and
Axio Scan.Z1, Carl Zeiss Microscopy).
Rajaram S., Heinrich L.E., Gordan J.D., Avva J., Bonness K.M., Witkiewicz A.K., Malter J.S., Atreya C.E., Warren R.S., Wu L.F, & Altschuler S.J. (2017). Sampling to capture single-cell heterogeneity. Nature methods, 14(10), 967-970.
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4

Visualizing KRAS RNA Expression

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KRAS RNA was detected using the RNAscope® 2.5 LS Probe for Hs-KRAS-O1 (ACD Biotechne cat. # 522968) in a Bond RX autostainer (Leica Biosystems) following the manufacturer’s directions. TSA-Cy5 and DAPI were used to visualize RNAScope signal and nuclei, respectively. Slides containing RNAscope fluorescently labeled cells were scanned either in the Aperio ScanScope FL or the Aperio Versa Digital Pathology Scanner using a 20X objective (Leica Biosystems). Images were archived in TPL’s eSlide Manger database (Leica Biosystems). Images were manually annotated for regions of interest using Tissue Studio software (Definiens Inc.Tissue Studio version 2.7 with Tissue Studio Library version 4.4.2). See supplementary methods for a detailed protocol.
Goulet D.R., Foster JP I.I., Zawistowski J.S., Bevill S.M., Noël M.P., Olivares-Quintero J.F., Sciaky N., Singh D., Santos C., Pattenden S.G., Davis I.J, & Johnson G.L. (2020). Discrete Adaptive Responses to MEK Inhibitor in Subpopulations of Triple-Negative Breast Cancer. Molecular cancer research : MCR, 18(11), 1685-1698.
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5

Multimodal Imaging of HCC Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
A collection of fresh frozen hepatocellular carcinoma (HCC, n=38) samples was used in the
present study. HCC specimens were collected at the University of California, San Francisco (San Francisco, CA). Institutional
Review Board approval was obtained and informed consent was obtained from all subjects. See Gordan et al., manuscript
in preparation, for further details of this sample set. Immunofluorescence staining and imaging was performed at
the Gladstone Institutes Histology and Light Microscopy core using fluorescently-labeled primary antibodies (all from Cell
Signaling, Danvers, MA) to CTNNB1 (Mouse mAb L54E2, conjugated to Alexa Fluor® 555, 1:200, Cat#5612) YAP
(Rabbit mAb D8H1X, conjugated to Alexa Fluor® 488, 1:200, Cat#14729) and LKB1 (Rabbit mAb, 1:250,
Cat#13031, followed by goat anti-rabbit conjugated to Alexa Fluor® 633, 1:200). Staining and imaging for
DAPI/YAP/LKB1 was first performed on 38 samples, and 5 months later serial sections for 25 of these samples were
stained/imaged for DAPI/YAP/CTNNB1. Stitched images of entire sections were acquired at 20× (0.32μm per pixel)
on automated slide scanners (BZ-X700; Keyence, Osaka, Japan; Aperio VERSA Digital Pathology Scanner, Leica Biosystems, and
Axio Scan.Z1, Carl Zeiss Microscopy).
Rajaram S., Heinrich L.E., Gordan J.D., Avva J., Bonness K.M., Witkiewicz A.K., Malter J.S., Atreya C.E., Warren R.S., Wu L.F, & Altschuler S.J. (2017). Sampling to capture single-cell heterogeneity. Nature methods, 14(10), 967-970.
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6

Image Acquisition and Analysis for IHC and IF

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IHC Stained slides were scanned using Aperio VERSA Digital Pathology Scanner (Leica Biosystems, Wetzlar, Germany). Images were acquired using ImageScope software (Leica Biosystems).
IF Images were acquired using the confocal microscope Leica TCS SP5 (Leica Biosystems, Germany). After filtration for background staining using slides stained with secondary-only antibodies, no further filtration or change of microscope setting was done through-out the image capture process. Images were export into TIFF files using the Fiji software18 (version 1.51 W), with no change of filters. Caspase-3 fluorescence intensity was calculated using the Imaris software (Oxford Instruments, UK, version 9.3.0).
Baruch E.N., Ortenberg R., Avivi C., Anafi L., Dick-Necula D., Stossel C., Moshkovits Y., Itzhaki O., Besser M.J., Schachter J., Barshack I, & Markel G. (2020). Immune co-culture cell microarray – a feasible tool for high-throughput functional investigation of lymphocyte–cancer interactions. Oncoimmunology, 9(1), 1741267.
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7

Multifluorescent Imaging of Protein Localization

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Immunofluorescent staining was performed with the PerkinElmer Opal kit (PerkinElmer). Briefly, thick formalin-fixed paraffin-embedded sections of 5-μm, baked and deparaffinized, were processed for antigen retrieval, blocking, primary and secondary antibody incubations, and signal amplification according to the manufacturer’s protocol. RGS14 (catalog no.: 16258-1; Proteintech; 1:200 dilution) was visualized using Opal 520 (green), and NHERF1 (EBP50; catalog no.: ab3452; Abcam; 1:200 dilution) was visualized using Opal 570 (red).
Fluorescent images were acquired using the Aperio Versa Digital Pathology Scanner (Leica Biosystems). The Versa scanner is based on a Leica DM6000B microscope with motorized stage and autofocus capabilities. Slides were scanned at 40× objective magnification with the 4′,6-diamidino-2-phenylindole, FITC, and tetramethylrhodamine filters. Optimal exposure times were determined before automated scanning.
Friedman P.A., Sneddon W.B., Mamonova T., Montanez-Miranda C., Ramineni S., Harbin N.H., Squires K.E., Gefter J.V., Magyar C.E., Emlet D.R, & Hepler J.R. (2022). RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein. The Journal of Biological Chemistry, 298(5), 101836.
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