Brains were removed, fixed in 10% formalin and embedded in paraffin. Four-micron coronal brain sections were stained with “Leica Bond Rx” (Leica Biosystems, Buffalo Grove, IL). Slides undergoing GFP analyses were pretreated with H2 antigen retrieval buffer for 35 min. Endogenous peroxidase was blocked with 3% hydrogen peroxide for 5 min. A TCT protein block was applied for 10 min (0.05 M Tris, 0.15 M NaCl, 0.25% Casein, 0.1% Tween 20, pH 7.6) and incubated with Anti-GFP antibody (1:1000, Invitrogen; A11122) for 60 min. All antibodies were then detected using Leica Power Vision HRP rabbit-specific polymer (Leica Biosystems; DS9800) for 12 min. Nuclear-specific staining was visualized with “Refine DAB” (Leica Biosystems; DS9800). A hematoxylin counterstain was then applied. Isotype negative control antibody-exposed slides were included as controls.
Refine dab
Manufactured by Leica
Refine DAB is a high-quality reagent used in immunohistochemistry (IHC) for the visualization of target proteins in tissue sections. It provides a sensitive and specific chromogenic detection system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using refine dab
1
Immunohistochemical Analysis of GFP Expression
2
Immunohistochemical Analysis of Lymph Nodes
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