O phosphoric acid

All chemicals used in the experiments were of analytical grade. Folin-Ciocalteu reagent, sodium carbonate, gallic acid, 1,1-DiPhenyl-2- 113 Picryl Hydrazyl (DPPH), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid 114 (Trolox), were purchased from Sigma-Aldrich (Darmstadt, Germany). FRAP, CUPRAC and proanthocyanidin assay kits were provided from Bioquochem, (Asturias, Spain). Tween 20 (Polyoxyethylene-20) was purchased from Biotech, (Markham, ON, Canada).
All HPLC standards, gallic acid, protocatechuic acid, (+)-catechin, chlorogenic acid, caffeic acid, p-coumaric acid, rutin, D-malic acid, myricetin, trans-cinnamic, 3,5-dimethoxy-4-hydroxycinnamic acid, quercetin, luteolin and kaempferol were purchased from Sigma-Aldrich (Darmstadt, Germany). Water was purchased from LiChrosolv^® (Darmstadt, Germany), O-phosphoric acid 85% extra pure was provided by Scharlau, Turkey and acetonitrile was provided by VWR Prolabo^® Chemicals (France).
A commercial cream, mostly containing water, glycerin, stabilizers, and preservatives with no bioactive molecules, was locally purchased from a store. A commercial hydro-glyceric extract obtained from grape seed was kindly provided by a cosmetics society in France.

Standard Ciprofloxacin hydrochloride was purchased from Sigma-Aldrich (Munich, Germany). O-phosphoric acid (85%) and triethylamine (99.5%) were purchased from Scharlau, Spain. HPLC-grade methanol and acetonitrile were obtained from Scharlau, Spain. Other chemicals used in this study were of higher analytical grade and purity.