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The F2442 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform specific functions within a laboratory setting. The core function of this product is to provide a controlled environment for various scientific applications. Further details about the intended use or specific applications of this product are not available.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (10 mentions) Fetal bovine serum (fbs), by Merck Group (3 mentions) Glass bottom dishes, by Ibidi (2 mentions) Glutamax and 4.5 g l glucose, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Sodium pyruvate, by Merck Group (2 mentions) Cc 2565, by Lonza (2 mentions) B 27 serum free supplement, by Thermo Fisher Scientific (2 mentions) Dmem f 12 nutrient mix, by Thermo Fisher Scientific (2 mentions) N2 supplement, by Thermo Fisher Scientific (2 mentions) Gentamicin, by Merck Group (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Bp9704100, by Thermo Fisher Scientific (1 mentions) P9767, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions) M4655, by Merck Group (1 mentions) Puromycin, by Corning (1 mentions)

Spelling variants of this product

F2442-500ML (3 citations)

14 protocols using f2442

1

Culturing Glucose-Responsive Min6 Insulinoma Cells

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We used glucose-responsive Min6 insulinoma cells [14 (link)] cultured in Dulbecco modified Eagle medium (DMEM; Invitrogen 41965-039) containing 15% fetal bovine serum (FBS; Sigma-Aldrich F2442), penicillin-streptomycin (ThermoFisher 15140122) and 0.05 mM 2mercaptoethanol (Gibco 31350010) at 37 °C and 5% CO2. The cell line has not been authenticated, but tested negative for mycoplasma by a polymerase chain reaction (PCR)-based detection kit (Sigma-Aldrich D9307). Medium containing 0.5 mM palmitate was prepared by first dissolving FFA-free BSA (Fisher Scientific BP9704100) in Min6 media to a final concentration of 0.6 M, then adding sodium palmitate (Sigma-Aldrich P9767). The solution was shaken overnight at 250 rpm at 50°C, and filtered before use.
Fan J., Du W., Kim-Muller J.Y., Son J., Kuo T., Larrea D., Garcia C., Kitamoto T., Kraakman M.J., Owusu-Ansah E., Cirulli V, & Accili D. (2020). Cyb5r3 links FoxO1-dependent mitochondrial dysfunction with β-cell failure. Molecular Metabolism, 34, 97-111.
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2

Imaging of Immunolabeled and Mitochondrial Dynamics

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For imaging immunolabeled samples, U2OS cells (human bone osteosarcoma, HTB-96, ATCC) were grown in McCoy’s 5A medium (ATCC) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, F2442) and 1% penicillin-streptomycin (ThermoFisher), and seeded on coverslips 2–3 days before experiments. For imaging mitochondria dynamics, HeLa cells29 (link) were grown in Dulbecco’s Modified Eagle Medium (DMEM) with glutaMAX and 4.5 g/L glucose (ThermoFisher), 1% (v/v) penicillin-streptomycin (Sigma-Aldrich), 1 mM sodium pyruvate (Sigma-Aldrich), and 10% (v/v) FBS (Merck Millipore) at 37°C in a 5% CO2 incubator. The cells were seeded in glass-bottom dishes (ibidi GmbH) one day prior to imaging.
Ebrahimi V., Stephan T., Kim J., Carravilla P., Eggeling C., Jakobs S, & Han K.Y. (2023). Deep learning enables fast, gentle STED microscopy. bioRxiv.
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3

Immunolabeling and Mitochondria Imaging

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For imaging immunolabeled samples, U2OS cells (human bone osteosarcoma, HTB-96, ATCC) were grown in McCoy’s 5 A medium (ATCC) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, F2442) and 1% penicillin-streptomycin (ThermoFisher), and seeded on coverslips 2-3 days before experiments. For imaging mitochondria dynamics, HeLa35 (link) or COS-7 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with glutaMAX and 4.5 g/L glucose (ThermoFisher), 1% (v/v) penicillin-streptomycin (Sigma-Aldrich), 1 mM sodium pyruvate (Sigma-Aldrich), and 10% (v/v) FBS (Merck Millipore) at 37 °C in a 5% CO2 incubator. The cells were seeded in glass-bottom dishes (ibidi GmbH) one day prior to imaging.
Ebrahimi V., Stephan T., Kim J., Carravilla P., Eggeling C., Jakobs S, & Han K.Y. (2023). Deep learning enables fast, gentle STED microscopy. Communications Biology, 6, 674.
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4

Culturing and Transfecting HeLa Cells

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HeLa cells were maintained in DMEM, GlutaMAX Supplement (Gibco, catalog 10569-010), supplemented with 10% (vol/vol) FBS (Sigma, catalog F2442) and the antibiotics penicillin and streptomycin (100 U/mL) (Gibco, catalog 15140-122) at 37oC with 5% CO2. HeLa cells were transfected with Lipofectamine 3000 Transfection Reagent (Invitrogen, catalog L3000015).
Yang H., Brown RH J.r., Wang D., Strauss K.A, & Gao G. (2023). Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery. JCI Insight, 8(9), e168688.
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5

Quantifying Senescent Fibroblast Dynamics

Cited 2 times
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The human diploid fibroblast WI-38 cell line (American Type Culture Collection, ATCC® CCL75™) was cultured in Minimum Essential Medium Eagle (Sigma-Aldrich, M4655) containing 10% FBS (Sigma-Aldrich, F2442), 1× Non-Essential Amino Acids Solution (Gibco, 11140050), 1 : 100 L-Glutamine–Penicillin–Streptomycin solution (Sigma-Aldrich, G6784), and maintained in a 37°C, 5% CO2 incubator. WI-38 fibroblasts were cultured from a cumulative population doubling (CPD) level of 37 representing a proliferative state based on Sidler et al. [20 (link), 21 (link)], to a senescent state defined by a failure to double after one week (CPD 56-60) [6 (link)]. Serial culture of WI-38 was performed by seeding cells at a density of 7000 cells/cm2 and trypsinization with 0.25% (weight/volume) Trypsin-0.53 mM EDTA solution every 3-4 days until senescent status was reached. Cell numbers were recorded for estimated CPD and doubling time. Doubling time was calculated as follows: Doubling time = duration∗log2 (2)/log2 (Final Cell Concentration)–log2 (Initial Cell Concentration) [22 ].
Chen Y.H., Zhang X., Ko K.Y., Hsueh M.F, & Kraus V.B. (2022). CBX4 Regulates Replicative Senescence of WI-38 Fibroblasts. Oxidative Medicine and Cellular Longevity, 2022, 5503575.
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6

Differentiation and Co-culture of Human iPSCs, ESCs, and Astrocytes

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Human iPSCs and ESCs were maintained on matrigel (Corning, 354230) dishes in mTesR (Stemcell Tech, 85850). For neuronal differentiation experiments, the medium was changed to neurogenic medium (DMEM/F-12 Nutrient Mix (GIBCO, 11320), 1x B-27 serum-free supplement (GIBCO, 17504), 1x N-2 supplement (GIBCO, 17502), and 25 μg/mL gentamicin (Sigma, G1397)). Human astrocytes (Lonza, CC-2565) were maintained in DMEM High Glucose supplemented with 10% FBS (Sigma, F2442) and 1% penicillin-streptomycin (GIBCO, 15140122) and transferred to neurogenic medium for co-culture with iPSC-derived neurons. For lentivirus production, HEK293T cells were cultured in DMEM High Glucose supplemented with 10% FBS and 1% penicillin-streptomycin.
Black J.B., McCutcheon S.R., Dube S., Barrera A., Klann T.S., Rice G.A., Adkar S.S., Soderling S.H., Reddy T.E, & Gersbach C.A. (2020). Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Cell reports, 33(9), 108460.
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7

HT29 Colorectal Cancer Cell Line Protocols

Cited 1 time
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The HT29 human colorectal carcinoma cell line (ATCC; HTB-38) was grown in McCoy’s 5A medium (Corning; 10-050-CV), with 10% fetal bovine serum (FBS) (Sigma-Aldrich; F2442) and 1% penicillin-streptomycin (Gibco; 15140-122). A cell clone from HT29 transduced in bulk with EFNB2-shRNA-596 lentivirus (clone 596-7) was selected with puromycin (Gibco; A11138-03; 3 μg/mL; 9 days) and maintained in tetracycline-free FBS (Corning; 35-075-CV) and 3 μg/mL puromycin. The monoclonality of clone 596-7 was verified by virus integration site mapping.27 (link)
Ha T., DiPrima M., Koparde V., Jailwala P., Ohnuki H., Feng J.X., Palangat M., Larson D, & Tosato G. (2022). Antisense transcription from lentiviral gene targeting linked to an integrated stress response in colorectal cancer cells. Molecular Therapy. Nucleic Acids, 28, 877-891.
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8

Equine Myeloid-Derived Immune Cells Protocol

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eMDMs were prepared from equine peripheral blood mononuclear cells as described previously [59 (link)], and maintained in RPMI 1640 (Sigma-Aldrich, R8758) supplemented with 30% horse serum (HyClone, SH30074.03) and 30% newborn bovine serum (Ausbian, VS500N). The human cell lines HEK293T and Hela were maintained in Dulbecco’s high-glucose modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Sigma-Aldrich, F2442) and 1% penicillin-streptomycin (Gibco, 15140122). U937 cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum. All primary cells and cultured cell lines were maintained at 37°C in a humidified incubator containing 5% carbon dioxide.
Ren H., Yin X., Su C., Guo M., Wang X.F., Na L., Lin Y, & Wang X. (2020). Equine lentivirus counteracts SAMHD1 restriction by Rev-mediated degradation of SAMHD1 via the BECN1-dependent lysosomal pathway. Autophagy, 17(10), 2800-2817.
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9

Differentiation and Co-culture of Human iPSCs, ESCs, and Astrocytes

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Human iPSCs and ESCs were maintained on matrigel (Corning, 354230) dishes in mTesR (Stemcell Tech, 85850). For neuronal differentiation experiments, the medium was changed to neurogenic medium (DMEM/F-12 Nutrient Mix (GIBCO, 11320), 1x B-27 serum-free supplement (GIBCO, 17504), 1x N-2 supplement (GIBCO, 17502), and 25 μg/mL gentamicin (Sigma, G1397)). Human astrocytes (Lonza, CC-2565) were maintained in DMEM High Glucose supplemented with 10% FBS (Sigma, F2442) and 1% penicillin-streptomycin (GIBCO, 15140122) and transferred to neurogenic medium for co-culture with iPSC-derived neurons. For lentivirus production, HEK293T cells were cultured in DMEM High Glucose supplemented with 10% FBS and 1% penicillin-streptomycin.
Black J.B., McCutcheon S.R., Dube S., Barrera A., Klann T.S., Rice G.A., Adkar S.S., Soderling S.H., Reddy T.E, & Gersbach C.A. (2020). Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Cell reports, 33(9), 108460.
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10

Neonatal Mouse Retinal Explant Preparation

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Mouse retinal explants were prepared as previously described [62]. Briefly, neonatal (postnatal day 0) pups were euthanized by CO2 asphyxiation, and eyes were enucleated and transferred to HBSS. The neural retina was dissected free from the retinal pigment epithelium (RPE), cut into small pieces (each ~3 mm2), and transferred into a 12-well cell culture plate. The retinal explants were maintained in a 1:1 mixture of DMEM and F12 (Gibco, 11320-033) containing 5% FBS (Sigma-Aldrich, F2442) and penicillin-streptomycin (Gibco, 15140122) for 24h at 37°C in an 8% CO2 atmosphere.
Yang H., Shen H., Li J, & Guo L.W. (2019). SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy, 15(9), 1539-1557.
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