The largest database of trusted experimental protocols

Anti ctsb

Manufactured by Proteintech
Sourced in United States

Anti-CTSB is a laboratory product that detects the presence of the Cathepsin B (CTSB) protein. CTSB is a cysteine protease involved in various cellular processes. This product can be used to measure CTSB levels in biological samples using techniques such as ELISA or Western blotting.

Automatically generated - may contain errors

2 protocols using anti ctsb

1

Comprehensive Autophagy Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The chemicals used in our experiments were: CA-4 (Selleckchem, Houston, TX, USA, S7204), CQ diphosphate (Selleckchem, S4430), and puromycin (Selleckchem, S7417), CHX (Medchem Express, NJ, USA, HY-12320), LysoTracker Red DND-99 (Invitrogen, Carlsbad, CA, USA, L-7258), LysoSensor Yellow/Blue DND-160 (Invitrogen, L-7545). The antibodies used in our experiments were: anti-MAP1LC3B/LC3B (Cell Signaling Technology, Beverly, MA, USA, 2775), anti-SQSTM1 (Cell Signaling Technology, 8025), anti-LAMP1 (Cell Signaling Technology, 9091), anti-NDRG1 (Cell Signaling Technology, 9485), anti-EGFR (Cell Signaling Technology, 4267), anti-p-AKT (Cell Signaling Technology, 13038), anti-p-ERK (Cell Signaling Technology, 9101), anti-cleaved PARP (Cell Signaling Technology, 5625), anti-CDKN1A/p21(Cell Signaling Technology, 2947), anti-CDKN1B/p27 (Cell Signaling Technology, 3686), anti-caspase 3 (Epitomics, Burlingame, CA, USA, 1087-1), anti-caspase 8 (Epitomics, 1007-1), anti-caspase 9 (Epitomics, 3392-1), anti-HIF-1α (Novus, Littleton, CO, USA, NB100-105), anti-CTSB (Proteintech, Rosemont, IL, USA, 12216-1-AP) and anti-CTSD (Proteintech, 21327-1-AP). Alexa Fluor 488- and 568-conjugated secondary antibodies were purchased from Invitrogen (A21429 and A11029).
+ Open protocol
+ Expand
2

Antibody panel for lysosomal studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study were as follows: anti-Rab7 (Santa Cruz Biotechnology, sc-376362), anti-Rab7 (Proteintech, 55469-1-AP), anti-p53 (Santa Cruz Biotechnology, sc-126), anti-SQSTM1/p62 (Proteintech, 18420-1-AP), anti-LC3B (MBL, M186-3), anti-RILP (Proteintech, 13574-1-AP), anti-GDI2 (Proteintech, 10116-1-AP), anti-LAMP1 (Proteintech, 55273-1-AP), anti-MON1A (Proteintech, 23772-1-AP), anti-CD63 (Proteintech, 25682-1-AP), anti-CTSB (Proteintech, 12216-1-AP), anti-CTSD (Proteintech, 21327-1-AP), anti-EEA1 (CUSABIO, CSB-PA007405LA01HU), Anti-EEA1 mAb-Alexa Fluor® 488 (MBL, M176-A48), anti-GAPDH (Proteintech, 60004-1-Ig), anti-MYC (Santa Cruz Biotechnology, sc-40), anti-FLAG (Biolegend, 637301), anti-GFP (Proteintech, 66002-1-Ig), anti-HA (Proteintech, 51064-2-AP), anti-GST (Proteintech, 10000-0-AP). Other reagents used in this study were: Hoechst 33342 (Sigma Aldrich, 14533), CQ (Sigma-Aldrich, C6628), Lyso-Tracker Red (Beyotime, C1046), and Lipofectamine® 2000 (Invitrogen, 11668019).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!