Log In Sign up
The largest database of trusted experimental protocols

E coli rosetta 2 de3 cells

Manufactured by Thermo Fisher Scientific
Visit Supplier

E. coli Rosetta 2 (DE3) cells are a genetically modified strain of E. coli bacteria commonly used in molecular biology and protein expression experiments. They are designed to enhance the expression of eukaryotic proteins by providing tRNAs that are rare in E. coli, improving the translation of proteins that contain codons not commonly found in E. coli.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Rosetta II (DE3) (8 citations) Rosetta™2 (DE3) cells (2 citations)

2 protocols using e coli rosetta 2 de3 cells

1

ΔFosB/FosB Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ΔFosB/FosB basic leucine zipper domain (FB, residues E153-K219) was sub-cloned into a pET21-NESG expression vector using the Mus musculus FosB cDNA and encodes the identical amino acid sequence as that in humans. The constructs have an N-terminal hexa-histidine (His) tag followed by a tobacco etch virus (TEV) protease cleavage site. Plasmids verified by DNA sequencing were transformed into E. coli Rosetta 2 (DE3) cells (Invitrogen).
Yin Z., Venkannagari H., Lynch H., Aglyamova G., Bhandari M., Machius M., Nestler E.J., Robison A.J, & Rudenko G. (2019). Self-assembly of the bZIP transcription factor ΔFosB. Current Research in Structural Biology, 2, 1-13.
+ Open protocol
+ Expand
2

Recombinant Expression of ΔFosB Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ΔFosB/FosB basic leucine zipper domain (FB, residues E153-K219) was sub-cloned into a pET21-NESG expression vector using the Mus musculus FosB cDNA and encodes the identical amino acid sequence as that in humans. The constructs have an N-terminal hexa-histidine (His) tag followed by a tobacco etch virus (TEV) protease cleavage site. Plasmids verified by DNA sequencing were transformed into E. coli Rosetta 2 (DE3) cells (Invitrogen).
Yin Z., Venkannagari H., Lynch H., Aglyamova G., Bhandari M., Machius M., Nestler E.J., Robison A.J, & Rudenko G. (2019). Self-assembly of the bZIP transcription factor ΔFosB. Current Research in Structural Biology, 2, 1-13.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!