Brightfield images of lungs from in vivo and in vitro experiments were captured either on a Leica MZ 125 stereoscopic dissecting microscope using a Spot Insight 2.0 Mp Color Mosaic camera and Spot 4.5.9 imaging software, or were obtained from live imaging experiments using a Leica DM6000B inverted microscope, DFC 305FX camera, and Leica Application Suite Advanced Fluorescence imaging software.
Application suite advanced fluorescence imaging software
Manufactured by Leica
The Application Suite Advanced Fluorescence imaging software is a comprehensive tool for the analysis and processing of fluorescence microscopy data. It provides a wide range of functionalities for image acquisition, visualization, and quantification, supporting a variety of microscopy techniques and applications.
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Lab products found in correlation
2 protocols using application suite advanced fluorescence imaging software
1
Brightfield Imaging of Lung Samples
2
Quantifying Lung Branching Dynamics
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Brightfield images of lungs from in vivo and in vitro experiments were captured either on a Leica MZ 125 stereoscopic dissecting microscope using a Spot Insight 2.0 Mp Color Mosaic camera and Spot 4.5.9 imaging software, or were obtained from live imaging experiments using a Leica DM6000B inverted microscope, DFC 305FX camera, and Leica Application Suite Advanced Fluorescence imaging software. Fluorescence intensities were quantified using FIJI software (version 2.0.0-rc-68/1.52 g).
Branching was quantified by counting and averaging the distal tips of the left lobe of control and experimental samples at 0 and 24 h. These groups were analyzed using a two-factorial quasi-Poisson model, with the dispersion parameter estimated as 0.317. Differences were considered statistically significant at p < 0.05.
Branching was quantified by counting and averaging the distal tips of the left lobe of control and experimental samples at 0 and 24 h. These groups were analyzed using a two-factorial quasi-Poisson model, with the dispersion parameter estimated as 0.317. Differences were considered statistically significant at p < 0.05.
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