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2 protocols using diosmin

1

Phytochemical Compound Characterization

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Epicatechin, kaempferol, quercetin, rutin, isoquercitrin, syringic acid, p-coumaric acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, ferulic acid and gallic acid were purchased from Adamas Reagent, Ltd. (Shanghai, China). Other standard reference materials including 4-methoxysalicylic acid, p-hydroxycinnamic acid, isoferulic acid, 1,3-dicaffeoylquinic acid, gentiopicrin, grosvenorine, diosmin, isorhamnetin and baicalein were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). 2,4,6-tri(pyridin-2-yl)-1,3,5-triazine (TPTZ), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 4-nitrophenyl α-D-glucopyranoside (p-NPG) were all obtained from Adamas Reagent, Ltd. (Shanghai, China). Acetonitrile and formic acid (99.9%, HPLC grade) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Cellulase (400 u/mg) from Trichoderma Vride G, hemiCellulase (20,000 u/g) and pectinase (500 u/mg) from Aspergillus niger were all supplied from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other reagents and chemicals used in this study are analytical grade.
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2

Comprehensive Authentication of Herbal Compounds

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There were
nine reference standards (including hesperidin, scopoletin, 5,6 dimethoxycoumarin,
5,7-dimethoxycoumarin, ferulic acid, diosmin, and vicenin), which
were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai,
China). Their purity was above 98% as tested using HPLC. Their identities
were confirmed by NMR spectroscopic methods and HR ESI-MS in a separate
publication. Methanol (MeOH) was bought from Chengdu Kelon chemical
reagent factory (Chengdu, China). The MS grade solvents including
acetonitrile (ACN) and formic acid (FA) were purchased from Sigma
(St. Louis, MO, USA).
The plant materials were collected from
seven different geographical regions in China and authenticated by
Prof. Yingjiao Zhang. Fresh materials were dried by the following
method. After air-drying, the sample was dried at −60 °C
at a vacuum of 0.1 kPa using an FD-1D-50 freeze-dryer (Beijing Bilang
Experimental Equipment Co., Ltd., Beijing, China). The dried slices
were milled into fine powder and stored at −18 °C for
the preparation of tests.
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