Staphylococcus aureus Rosenbach (S. aureus, gram-positive, ATCC 14458) and Pseudomonas aeruginosa (Schroeter) Migula (P. aeruginosa, gram-negative, ATCC 10145) and were purchased from American Type Culture Collection (ATCC, Manassas, VA) and used by following the supplier’s protocol. Briefly, bacteria were kept at −80°C. Prior to use, bacteria were grown in 100 mL of Bacto Agar medium (Becton Dickinson) overnight. Then 1 mL of bacteria containing medium was applied to 100 mL of fresh agar medium for 2 hrs. Bacteria were then spun down at 4,500 rpm for 10 min at 4°C. Finally, bacteria were resuspended in sterile PBS to OD600=0.1. This protocol results in an inoculum of 5×106 CFU in 50 μL (as confirmed by direct count).
Pseudomonas aeruginosa schroeter migula
Manufactured by American Type Culture Collection
Sourced in Italy
Pseudomonas aeruginosa (Schroeter) Migula is a gram-negative, aerobic bacterium. It is a type strain that can be used for various microbiological studies and applications.
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2 protocols using pseudomonas aeruginosa schroeter migula
1
Preparation of Bacterial Inoculum
2
Cultivation of Oral Bacterial Strains
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Streptococcus mutans Clarke (strain designation NCTC 10449), Pseudomonas aeruginosa (Schroeter) Migula (strain designation PAO1), Fusobacterium nucleatum, subspecies nucleatum (strain VPI 4355), and Porphyromonas gingivalis (strain 2561) were purchased from ATCC (LGC Standards Srl, Milan, Italy). Streptococcus mitis, Streptococcus oralis, and Streptococcus sanguinis were obtained from clinical isolates and characterized by morphological, biochemical, and molecular typing. S. mutans, S. mitis, S. oralis, and S. sanguinis were maintained in Brain Heart Infusion (BHI) broth or agar under microaerophilic conditions. P. aeruginosa was maintained in trypticase soy (TS) agar or broth. F. nucleatum was maintained in TS agar or broth with defibrinated sheep blood under anaerobic conditions. P. gingivalis was maintained in ATCC Medium 2722 supplemented with TS broth or agar under anaerobic conditions. Before the experiments, bacterial cultures were inoculated in fresh media (dilution 1:100) and grown for 16 h at 37 °C.
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