Blood specimens were collected after a 12- to 14-hour fast (8 pm to 9.30 am) to reduce the influence of circadian variation. Total cholesterol and triglyceride concentrations were measured using standard enzyme methods. High-density lipoprotein cholesterol was measured after precipitation of very-low-density lipoproteins and low-density lipoprotein with phosphotungstic acid, and low-density lipoprotein was calculated using the Friedewald formula. Fasting glucose levels were enzymatically determined by the hexokinase method.11 (link) A blood sample from every patient was drawn and centrifuged within 30 minutes; serum samples were stored at −80°C, and high sensitivity C-reactive protein was determined using an immunoturbidity assay system (Liatest; Stago, Asnières-sur-Seine, France), with an interassay variability coefficient of variation of 6.25%.12 (link)
Liatest
Manufactured by Stago
Sourced in France
Liatest is a diagnostic laboratory instrument designed for the measurement of coagulation parameters. It utilizes automated techniques to perform quantitative analysis of various coagulation factors and markers. The core function of Liatest is to provide accurate and reliable data for the assessment of the blood coagulation system.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using liatest
1
Fasting Biomarker Measurement Protocol
2
Fasting Lipid and Glucose Analysis
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Blood specimens were obtained after a 12- to 14-hour fast (8 pm–9.30 am) to reduce the influence of circadian variation. The concentrations of total cholesterol and triglyceride were measured using standard enzyme methods. High-density lipoprotein (HDL) cholesterol was assessed after very LDL with phosphotungstic acid precipitation, and LDL was calculated using the Friedewald formula. Fasting glucose levels were enzymatically examined by the hexokinase method.9 (link) A blood sample was collected from every patient and centrifuged within 30 minutes; the serum samples were stored at −80°C, and high-sensitivity C-reactive protein was measured using an immunoturbidity assay system (Liatest; Stago, Asnières-sur- Seine, France), with an interassay variability coefficient of variation of 6.25%.10 (link)
3
Fasting Blood Lipid and Glucose Profiling
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Blood specimens were collected after a 12- to 14-hour fast (8 pm to 9.30 am) to reduce the influence of circadian variation. Total cholesterol and triglyceride concentrations were measured using standard enzyme methods. High-density lipoprotein cholesterol was measured after precipitation of very-low-density lipoproteins and low-density lipoprotein with phosphotungstic acid, and low-density lipoprotein was calculated using the Friedewald formula. Fasting glucose levels were enzymatically determined by the hexokinase method. A blood sample from every patient was drawn and centrifuged within 30 minutes. The serum samples were stored at −80°C, and high-sensitivity C-reactive protein was determined using an immunoturbidity assay system (Liatest; Stago, Asnières-sur-Seine, France), with an interassay variability coefficient of variation of 6.25%.
4
Lipid and Glucose Profile Analysis
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Blood specimens were obtained after a 12 to 14-h fast (8:00 p.m.–9:30 a.m.) to reduce the influence of circadian variation. Total cholesterol (TC) and triglyceride (TG) concentrations were assessed by using standard enzyme methods. The high-density lipoprotein (HDL) cholesterol level was measured after precipitation of very-low-density lipoproteins and low-density lipoprotein (LDL) with phosphotungstic acid, and LDL was calculated using the Friedewald formula. Fasting glucose levels were enzymatically measured by the hexokinase method. A blood sample from every patient was drawn and centrifuged within 30 min. The serum samples were stored at −80°C, and high sensitivity C-reactive protein (hs-CRP) was determined using an immunoturbidity assay (Liatest; Stago, Asnieres-sur-Seine, France), with an interassay variability coefficient of variation of 6.25%. The above method proceeded in the same way as in our previous study (15 (link)).
5
Lipid Profile and Inflammation Assessment
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Blood specimens were obtained after a 12- to 14-h fast (8:00 PM to 9:30 AM) to reduce the influence of circadian variation. Total cholesterol [17 ] and triglyceride [8 (link)] concentrations were assessed using standard enzymatic methods. High-density lipoprotein (HDL) cholesterol level was measured after precipitation of very-low-density lipoproteins and low-density lipoproteins (LDL) with phosphotungstic acid, and LDL was calculated using the Friedewald formula. Serum samples were stored at –80 °C, and high-sensitivity C-reactive protein was determined using an immunoturbidity assay (Liatest; Stago, Asnieres-sur-Seine, France), with a 6.25% interassay variability coefficient.
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