For microscopic observations, an Olympus System microscope Model BX51 (Olympus) equipped with UPlanSApo 60× and UPlanFL 100× objective lenses (Olympus, Shinkjuku, Japan) and a stereomicroscope Model SMZ800 (Nikon, Minato, Japan) were used. Images were captured with a DP71 digital camera (Olympus) and processed using DP manager imaging software (Olympus, Shinkjuku, Japan).
System microscope model bx51
Manufactured by Olympus
Sourced in Japan
The Olympus System Microscope Model BX51 is a high-performance microscope designed for laboratory and research applications. It features a sturdy and ergonomic design with a range of optical and illumination options to accommodate various specimen types and observation techniques.
Automatically generated - may contain errors
Lab products found in correlation
Dp71 digital camera, by Olympus (4 mentions)
Dp manager imaging software, by Olympus (4 mentions)
Uplansapo 60, by Olympus (2 mentions)
Hoechst 33342, by Merck Group (2 mentions)
Model smz800, by Nikon (2 mentions)
Uplansapo 60x, by Olympus (2 mentions)
Uplanfl 100, by Olympus (1 mentions)
Xcd sx90cr, by Sony (1 mentions)
H 1000, by Vector Laboratories (1 mentions)
Ex300, by Olympus (1 mentions)
6 protocols using system microscope model bx51
1
Microscopic Observation Protocol
2
Microscopic Imaging of Fungal Hyphae
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For microcopy, an Olympus System microscope Model BX51 (Olympus) equipped with UPlanSApo 60X and UPlanFL 100X objective lenses (Olympus) and stereomicroscope Model SMZ800 (Nikon) were used. Images were captured with a DP71 digital camera (Olympus) and processed using the DP manager imaging software (Olympus). For microscopic observation of the fungal hyphae, each strain was coverslip-cultured on a block of appropriate agar medium or incubated in liquid GMM medium. The coverslips were stained with 1 mg/ml Hoechst 33342 (Sigma-Aldrich) for labeling DNA2 (link). DAPI (high brightness) filter cubes (excitation filter: center wavelength 377 nm, emission filter: center wavelength 447 nm, Olympus) and FITC filter cubes (excitation filter: center wavelength 483 nm, emission filter: center wavelength 535 nm, Olympus) were used to observe the fluorescence of Hoechst and YFP, respectively50 (link).
3
Polarized Optical Microscopy of Samples
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Polarized optical microscopy (POM) was performed using an upright Olympus System Microscope Model BX51, with crossed polarizers and a 530 nm retardation plate (U-TP530).
Digital images were taken of samples between the microscopy slide and the cover glass using a Sony XCD-SX90CR charge-coupled device camera. The microscope was equipped with a HS82 heated stage (METTLER TOLEDO) allowing temperature control under the microscope.
Digital images were taken of samples between the microscopy slide and the cover glass using a Sony XCD-SX90CR charge-coupled device camera. The microscope was equipped with a HS82 heated stage (METTLER TOLEDO) allowing temperature control under the microscope.
4
Coverslip Culture and Fluorescence Microscopy
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Coverslip culture was performed as described previously36 (link). The coverslips were stained with 1 mg/ml Hoechst 33342 (Sigma) for 10 min, briefly washed with distilled water and dipped in distilled water for 10 minutes. Then the coverslips were washed with ethanol, air-dried for 5 minutes and mounted with antifade mounting medium (H-1000; Vectashield, USA). For differential interference contrast (DIC) and fluorescence microscopy, an Olympus System microscope Model BX51 (Olympus, Japan) equipped with UPlanSApo 60× and UPlanFL 100× objective lenses (Olympus) were used. DAPI (High brightness) filter cube (Excitation filter: center wavelength 377 nm, Emission filter: center wavelength 447 nm; Olympus) and FITC filter cube (Excitation filter: center wavelength 483 nm, Emission filter: center wavelength 535 nm; Olympus) were used to observe the fluorescence of Hoechst and YFP, respectively. Images were captured with a DP71 digital camera (Olympus) and processed using the DP manager imaging software (Olympus) and Photoshop CS5.1 (Adobe Systems, USA).
5
Microscopy Imaging and Digital Capture
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For microcopy, the Olympus System microscope Model BX51 (Olympus) equipped with UPlanSApo 60X and UPlanFL 100X objective lenses (Olympus) and a stereomicroscope Model SMZ800 (Nikon) were used. Images were captured with a DP71 digital camera (Olympus) and processed using the DP manager imaging software (Olympus).
6
Organogel Micrograph Imaging
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Micrographs of organogels (4 %w/w) were obtained under polarized light microscope Olympus System Microscope model BX 51 (Olympus America Inc. Center Valley, PA, USA) equipped with a digital camera model Olympus EX300 (Olympus America Inc. Center Valley, PA, USA).
Samples were melted at 80 °C and a small drop put over a heated glass slide and covered with a glass coverslip. The glass slides were crystallized on at 5 and 25°C for 24 hours and analyzed at 25°C. Samples were treated using Image cell Sens Standard 1.7.1.
(Olympus, USA).
Samples were melted at 80 °C and a small drop put over a heated glass slide and covered with a glass coverslip. The glass slides were crystallized on at 5 and 25°C for 24 hours and analyzed at 25°C. Samples were treated using Image cell Sens Standard 1.7.1.
(Olympus, USA).
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