To quantify the IGF-1/GLP-1 (Candeias et al., 2018 (link), Ola et al., 2014 (link)) and myelin basic protein (MBP) (Khan et al., 2006 (link)) levels in rat brain homogenates, a diagnostic kit (E-EL-R0010/ IGF-1; E-EL-R3007/GLP-1; E-EL-R0642/MBP; Elabsciences, Wuhan, Hubei, China) was used in the experimental protocol. As outlined in the kit, samples and all reagents were prepared with minor alterations according to the manufacturers' protocol. A Bio-Rad iMark 96-well plate reader was used to measure the absorbance at 450 nm. The results have been shown as nanogram per milligram protein.
Imark 96 well plate reader
Manufactured by Bio-Rad
Sourced in Canada, United States
The IMark 96-well plate reader is a laboratory instrument designed to measure the absorbance of samples in a 96-well microplate format. It is capable of collecting data across multiple wavelengths to support various analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
E el r0010 igf 1, by Elabscience (1 mentions)
E el r3007 glp 1, by Elabscience (1 mentions)
E el r0642 mbp, by Elabscience (1 mentions)
Quantikine elisa kit for human vegf, by R&D Systems (1 mentions)
Kaleidagraph v4, by Synergy Software (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Cremophor el, by Maclin Biochemical Technology (1 mentions)
Peg 400, by Maclin Biochemical Technology (1 mentions)
β glucuronidase, by Merck Group (1 mentions)
Avance 3 hd 400 mhz digital nmr spectrometer, by Bruker (1 mentions)
Tween 80, by Maclin Biochemical Technology (1 mentions)
5 protocols using imark 96 well plate reader
1
Quantitative Analysis of Brain Protein Biomarkers
2
Quantifying Platelet Granule Secretion
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As markers of platelet α- and δ-granule secretion, vascular endothelial cell growth factor (VEGF
165) and serotonin (5-HT) were quantified in platelet releasates using the Quantikine ELISA Kit for human VEGF (R&D Systems, Minneapolis, Minnesota, United States) and a Serotonin enzyme-linked immunosorbent assay (ELISA) kit (Enzo Life Sciences, Brockville, Ontario, Canada), respectively. ELISAs were performed according to manufacturer's instructions and absorbance measured using an iMark 96-well plate reader (Bio-Rad, Mississauga, Ontario, Canada). The effects of warfarin on platelet secretion of VEGF and 5-HT were then expressed as percent of vehicle (saline) control.
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As markers of platelet α- and δ-granule secretion, vascular endothelial cell growth factor (VEGF
165) and serotonin (5-HT) were quantified in platelet releasates using the Quantikine ELISA Kit for human VEGF (R&D Systems, Minneapolis, Minnesota, United States) and a Serotonin enzyme-linked immunosorbent assay (ELISA) kit (Enzo Life Sciences, Brockville, Ontario, Canada), respectively. ELISAs were performed according to manufacturer's instructions and absorbance measured using an iMark 96-well plate reader (Bio-Rad, Mississauga, Ontario, Canada). The effects of warfarin on platelet secretion of VEGF and 5-HT were then expressed as percent of vehicle (saline) control.
3
Quantifying VCC-mediated Lysis of Rabbit Blood
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Purified VCC was activated by proteolytic cleavage using α-chymotrypsin (1:350 wt/wt) for 30 minutes at room temperature and serially diluted. Activated VCC dilutions were added to wells in a 96-well clear bottom plate containing defibrinated rabbit whole blood diluted to an absorbance at 595 nm of 1.0 in blood dilution buffer (20 mM sodium phosphate pH 7.4, 150 mM NaCl, 1mg/ml BSA). The absorbance was monitored at 595 nm every 15 seconds at room temperature in an iMark 96-well plate reader (Bio-rad Laboratories, Inc.). Raw absorbance data were converted into % lysis and HD50 values calculated as described previously [36 (link)] using KaleidaGraph v. 4.1.3 (Synergy Software).
4
Paclitaxel-Loaded Glucose Conjugate Synthesis
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Paclitaxel, succinate anhydride, 2,3,4,6-Tetra-O-benzyl-d -glucose, succinic anhydride, 4-dimethylaminopyridine (DMAP), dicyclohexylcarbodiimide (DCC), palladium on 10% activated carbon (10% Pd/C), chlorotriethylsilane (TESCl), tetrabutylammonium fluoride (TBAF), dichloromethane (DCM), tetrahydrofuran (THF), methanol (MeOH), acetic acid (AcOH), n-hexane (Hex), and ethyl acetate (EA) were purchased from Energy Chemicals (Shanghai, China). Cremophor EL, Tween-80 and PEG-400 were purchased from Macklin (Shanghai, China).
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium romide (MTT), dimethyl sulfoxide (DMSO), and β-glucuronidase were purchased from Sigma Aldrich (St. Louis, MO, USA). Leibovitz’s medium (L-15 medium), fetal bovine serum (FBS), and Hank’s balanced salt solution (HBSS) were purchased from Gibco (New York, NY, USA), rabbit serum (RS) was purchased from BestBio (Shanghai, China).
NMR spectra were recorded on a Bruker AVANCE III HD 400MHz Digital NMR Spectrometer (Karlsruhe, Germany). ESI mass spectra were recorded on a Thermo Scientific TSQ QuantumTM Access MAX Mass Spectrometer (Waltham, MA, USA). Optical Density (OD) of MTT assay was measured with a Bio-rad iMark 96-well plate reader (Hercules, CA, USA).
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium romide (MTT), dimethyl sulfoxide (DMSO), and β-glucuronidase were purchased from Sigma Aldrich (St. Louis, MO, USA). Leibovitz’s medium (L-15 medium), fetal bovine serum (FBS), and Hank’s balanced salt solution (HBSS) were purchased from Gibco (New York, NY, USA), rabbit serum (RS) was purchased from BestBio (Shanghai, China).
NMR spectra were recorded on a Bruker AVANCE III HD 400MHz Digital NMR Spectrometer (Karlsruhe, Germany). ESI mass spectra were recorded on a Thermo Scientific TSQ QuantumTM Access MAX Mass Spectrometer (Waltham, MA, USA). Optical Density (OD) of MTT assay was measured with a Bio-rad iMark 96-well plate reader (Hercules, CA, USA).
5
Rat Brain Apoptosis Protein Quantification
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For the measurement of Caspase-3 (Wang et al., 2002 (link)) and Bax (Tiwari et al., 2021 (link)), Bcl-2 (Bai et al., 2019 (link); Moneim, 2015 (link)) levels in rat brain homogenates, a diagnostic kit (E-EL-R0160/Caspase-3; E-EL-R0098/Bax/Bcl2Elabsciences, Wuhan, Hubei, China) was used. As outlined in the kit, all reagents and samples were prepared with minor alterations according to the manufacturers' protocol. A Bio-Rad iMark 96-well plate reader was used for 450 nm absorbance measurement. The results have been shown as nanogram per milligram protein.
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