Western blots were performed as previously described [48 (link),53 (link),55 (link)] in lysed mature and immature thyrocyte spheroids. The following primary antibodies were used at 1:1000 dilution: Anti-SLC7A11 (D2M7A) and antitubulin (D3U1W) (both from Cell Signaling Technology) and anti-PRDX1 (D5G12), antithyroglobulin (D-9), and anti-NQO1 (A180) (all from Santa Cruz).
Anti nqo1 a180
Manufactured by Santa Cruz Biotechnology
Anti-NQO1 (A180) is a primary antibody product offered by Santa Cruz Biotechnology. The antibody is designed to detect the expression of the NQO1 protein, which plays a role in cellular redox homeostasis. This product is suitable for various applications, including western blotting, immunohistochemistry, and immunocytochemistry, where the detection of NQO1 is of interest.
Automatically generated - may contain errors
Lab products found in correlation
Dasatinib, by Merck Group (1 mentions)
Trigonelline, by Merck Group (1 mentions)
Erastin, by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Anti p70s6k, by Cell Signaling Technology (1 mentions)
Anti gapdh d16h11, by Cell Signaling Technology (1 mentions)
Anti phospho p70s6k thr389, by Cell Signaling Technology (1 mentions)
Anti erk1 2 137f5, by Cell Signaling Technology (1 mentions)
Anti ubiquitin p4d1, by Santa Cruz Biotechnology (1 mentions)
Anti nrf2 d1z9c, by Cell Signaling Technology (1 mentions)
Anti vinculin e1e9v, by Cell Signaling Technology (1 mentions)
Ml385, by Selleck Chemicals (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Ferrostatin 1, by Merck Group (1 mentions)
Anti p62 sqstm1, by MBL Life Science (1 mentions)
2 protocols using anti nqo1 a180
1
Western Blot Analysis of Thyrocyte Spheroids
2
NRF2 Regulation via p62/SQSTM1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies used are as follows: anti-NRF2 (D1Z9C) (Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-vinculin (E1E9V) (Cell Signaling Technology), anti-lamin (E-1) (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (D16H11) (Cell Signaling Technology); anti-Src (2108S) (Cell Signaling Technology), anti-phospho-Src (Tyr416) (2101S) (Cell Signaling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-NQO1 (A-180) (sc-32793; Santa Cruz Biotechnology), anti-p70S6K (Cell Signaling Technology), anti-phospho-p70S6K (Thr389) (Cell Signaling Technology), anti-ERK 1/2 (137F5) (Cell Signaling Technology), anti-phospho-ERK 1/2 (Thr202/Tyr204) (D13.14.4E) (Cell Signaling Technology), anti-ubiquitin (P4D1; Santa Cruz Biotechnology).
Dasatinib, trigonelline, rapamycin, ferrostatin-1, erastin, and cycloheximide were purchased from Sigma-Aldrich; PP2 (sc-202769) from Santa Cruz Biotechnology; ML385 from Selleck Chemicals.
Dasatinib, trigonelline, rapamycin, ferrostatin-1, erastin, and cycloheximide were purchased from Sigma-Aldrich; PP2 (sc-202769) from Santa Cruz Biotechnology; ML385 from Selleck Chemicals.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!