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Ra2159

Manufactured by Cell Applications
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RA2159 is a laboratory instrument designed for cell culture applications. It provides basic functionality for maintaining and monitoring cell cultures. The core function of RA2159 is to facilitate controlled incubation of cell samples at specified temperature and humidity levels.

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Lab products found in correlation

Synoviocyte basal medium, by Cell Applications (2 mentions) Synoviocyte growth medium, by Cell Applications (2 mentions)

2 protocols using ra2159

1

Cultivation of Primary Human Synovial Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Normal primary human synovial fibroblasts (SF) (human fibroblast–like synovioctyes (HFLS), cat. no. 408–05a) and RA SF (HFLS–RA, cat. no. 408RA–05a) were purchased from Cell Applications, Inc (see Supplementary Table 2 for details). The nomenclature for donor–derived SF samples references specific lot number (e.g. N2586 is Cell Applications HFLS lot 2586, RA2159 is Cell Applications HFLS–RA lot 2159, etc). Cells were cultured according to the supplier’s recommendations using Synoviocyte Growth Medium (Cell Applications, Inc. cat. no. 415–500) as full growth medium. Synoviocyte Basal Medium (Cell Applications, Inc. cat. no. 414–500) was used for serum starvation prior to experimental treatments. Cells were provided at passage 2 and all experiments were conducted on cells at passage 3 – 6, in accordance with published recommendations.1
Jones D.S., Jenney A.P., Swantek J.L., Burke J.M., Lauffenburger D.A, & Sorger P.K. (2016). Profiling drugs for rheumatoid arthritis that inhibit synovial fibroblast activation. Nature chemical biology, 13(1), 38-45.
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2

Cultivation of Primary Human Synovial Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Normal primary human synovial fibroblasts (SF) (human fibroblast–like synovioctyes (HFLS), cat. no. 408–05a) and RA SF (HFLS–RA, cat. no. 408RA–05a) were purchased from Cell Applications, Inc (see Supplementary Table 2 for details). The nomenclature for donor–derived SF samples references specific lot number (e.g. N2586 is Cell Applications HFLS lot 2586, RA2159 is Cell Applications HFLS–RA lot 2159, etc). Cells were cultured according to the supplier’s recommendations using Synoviocyte Growth Medium (Cell Applications, Inc. cat. no. 415–500) as full growth medium. Synoviocyte Basal Medium (Cell Applications, Inc. cat. no. 414–500) was used for serum starvation prior to experimental treatments. Cells were provided at passage 2 and all experiments were conducted on cells at passage 3 – 6, in accordance with published recommendations.1
Jones D.S., Jenney A.P., Swantek J.L., Burke J.M., Lauffenburger D.A, & Sorger P.K. (2016). Profiling drugs for rheumatoid arthritis that inhibit synovial fibroblast activation. Nature chemical biology, 13(1), 38-45.
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