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Anti cd45 apc efluor780

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Anti-CD45-APC-eFluor780 is a fluorescently labeled antibody that binds to the CD45 cell surface antigen. CD45 is a protein tyrosine phosphatase that is expressed on the surface of all leukocytes and is involved in the regulation of cell growth and differentiation.

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Lab products found in correlation

Anti cd3 apc efluor780, by Thermo Fisher Scientific (1 mentions) Anti ifn γ apc, by BioLegend (1 mentions) Anti cd4 pacific blue, by BioLegend (1 mentions) Anti cd8α percp cy5, by BioLegend (1 mentions) Anti cd69 fitc, by BioLegend (1 mentions) Rat igg2b isotype antibody, by BioXCell (1 mentions) Anti cd44 alexa fluor 700, by BioLegend (1 mentions)

Spelling variants of this product

Anti-CD45 (228 citations) CD45-APC (97 citations) Anti-CD45-APC (42 citations)

2 protocols using anti cd45 apc efluor780

1

Multiparameter Analysis of CD8+ T Cells

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533IYSTVASSL541 (IVL533-541)-tetramer was bought from MBL (cat. NO.TS-M520-1), anti-CD8α depletion antibody (clone: YTS 169.4). Rat IgG2b isotype antibody (clone: LTF-2) was purchased from Bio X Cell. Antibodies for flow cytometry included anti-CD3-APC-eFluor780 (clone: 17A2; eBioscience), anti-CD4-Pacific Blue (clone: GK1.5; BioLegend), anti–CD8α-PerCP/Cy5.5 (clone: 53–6.7; BioLegend), anti-CD11a-PE/Cy7 (clone: 2D7; BD Bioscience), anti–CD44-Alexa Fluor 700 (clone: IM7; BioLegend), anti-CD45-APC-eFluor780 (clone:30-F11;Biolegend), anti-CD62L-eVolve 605 (clone: MEL-14; eBioscience), anti-CD127-PE & PE/Cy7 (clone: A7R34; BioLegend), anti-KLRG1-FITC & BV510 (clone: 2F1/KLRG1; BioLegend), anti-CD103-APC (clone: 2E7; BioLegend), anti-CD69-FITC (clone: H1.2F3; BioLegend) and anti-IFNγ-APC (clone: XMG1.2; BioLegend), anti-Eomes-PE & PE/Cy7 (clone: Dan11mag; eBioscience).
Zheng X., Oduro J.D., Boehme J.D., Borkner L., Ebensen T., Heise U., Gereke M., Pils M.C., Krmpotic A., Guzmán C.A., Bruder D, & Čičin-Šain L. (2019). Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge. PLoS Pathogens, 15(9), e1008036.
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2

Lymphocyte Isolation and Fluorescent Labeling

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Mice were intravenously (i.v.) injected with 3 μg anti-CD45-APC/eFluor780 (clone: 30-F11; BioLegend). Mice were euthanatized 3–5 min after injection, and blood, spleen and lungs were collected. Following their isolation from the respective compartment, lymphocytes were stained and analyzed as described above.
Zheng X., Oduro J.D., Boehme J.D., Borkner L., Ebensen T., Heise U., Gereke M., Pils M.C., Krmpotic A., Guzmán C.A., Bruder D, & Čičin-Šain L. (2019). Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge. PLoS Pathogens, 15(9), e1008036.
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