Construction of MV-m-uPA and MV-h-uPA, virus rescue, propagation and titration were performed as previously reported [24 (link), 36 (link)]. 4T1 cells (murine mammary carcinoma), MDA-MB-231 cells (human breast cancer), MCF-7 cells (human breast cancer), MDA-MB-436 cells (human breast cancer) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). 4T1-luc2 cells stably expressing highly efficient luciferase were from PerkinElmer (Santa Clara CA). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), penicillin and streptomycin at 37 °C and 5% CO2. HMEC cells (human mammary epithelial cells) were purchased from Lonza (Walkersville, MD) and maintained in MEGM according to manufacture’s instruction at 37 °C and 5% CO2. NMuMG (murine mammary epithelial cells) were purchased from the ATCC and maintained in DMEM containing 10% fetal bovine serum (FBS), penicillin and streptomycin at 37 °C and 5% CO2. Vero-αHis cells [36 (link)] were grown in DMEM containing 10% FBS at 37 °C and 5% CO2.
4t1 luc2 cells
Manufactured by PerkinElmer
Sourced in United States
4T1-luc2 cells are a bioluminescent mouse mammary carcinoma cell line. They express the luciferase reporter gene, enabling in vivo imaging of tumor growth and metastasis.
Automatically generated - may contain errors
Lab products found in correlation
Nmumg, by American Type Culture Collection (1 mentions)
Hmec cells, by Lonza (1 mentions)
Mcf 7 cell, by American Type Culture Collection (1 mentions)
4t1 cell, by American Type Culture Collection (1 mentions)
Mda mb 436 cells, by American Type Culture Collection (1 mentions)
B16f10 cells, by American Type Culture Collection (1 mentions)
Balb c mice, by Charles River Laboratories (1 mentions)
4 protocols using 4t1 luc2 cells
1
Virus-based Breast Cancer Cell Lines
2
Tumor Tracking with Fluorescent Probes
3
Murine Mammary Tumor Cell Lines
4
Murine Mammary Tumor Xenograft Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Eight-week old female BALB/c mice were purchased from Charles River Laboratories (Germany). Animals were housed 6e10 per cage under a light-dark (12 h/12 h) cycle with ad libitum access to water and food. Experimental manipulations were performed under the inhalation anesthesia induced by 4% and maintained by 2.3% mixture of isofluorane in oxygen administered through facial masks. Experiments were carried in compliance with the bioethical principles adopted by the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), and Ethical Committee for Animal Experiments of the North Stockholm region N66_13 and Latvian Animal Protection Ethics Committee of the Latvian Food and Veterinary Service, permission Nr 99. 4T1luc2 cells (Perkin Elmer) in 50 ml of serum free DMEM (Dulbecco's Modified Eagle's Medium, HyClone) were injected into the mammary fat pads of 9 week old BALB/c mice. Cells were implanted at two sites per mouse in serial five-fold dilutions from a starting concentration of 6.25*10 5 cells per 50 ml. After the experiments, mice were humanely sacrificed by cervical dislocation.
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