Cri camera system

To capture the hyperspectral image data, a CRi camera system (PerkinElmer, Hopkinton, MA) was used to acquire images from animals. The system is a light-tight apparatus that uses a Cermax-type 300 Watt Xenon light source. This provides lights that span the electromagnetic spectrum from 450–950 nm. The CCD is a 16-bit, high-resolution, scientific-grade imaging sensor. Four, fiber-optic, adjustable illuminator arms yield an even light distribution to the subject. The light radiates from the excitation source and then illuminates the sample. Reflect lights pass through the camera lens to the solid-state liquid crystal tuning element and finally to the CCD. The excitation and emission filter sliders hold two, 50-mm diameter, long pass filters. The long pass filters remove the band light especially from the excitation source. These filters are color coded to indicate the wavelength they represent. The field of view (length × width) is from 3.4×2.5 cm to 10.2 ×7.6 cm with variable zoom. The resolution is from 25 to 75 μm based on the zoom lens position. The fluence rate, i.e., the flow of photons over a set angle, is from 4 to 20 mW/cm2 based on the light position. The scan time is from 5 sec to 1 min.¹⁰ The images were then normalized using the method as previously reported by us.¹⁰

A CRi camera system (PerkinElmer, Hopkinton, MA) was used to acquire images from animals. The system is a light-tight apparatus that uses a Cermax-type 300 Watt Xenon light source. This provides lights that spans the electromagnetic spectrum from 450–900nm. The CCD is a 12-bit, high-resolution, scientific-grade imaging sensor. Four, fiber-optic, adjustable illuminator arms yield an even light distribution to the subject. The light radiates from the excitation source and then illuminates the sample. Reflected light passes through the camera lens to the solid-state liquid crystal tuning element and finally to the CCD. The field of view (length × width) is from 3.4×2.5 cm to 10.2 ×7.6cm with variable zoom. The resolution is from 25 to 75 µm based on the zoom lens position. The scan time is from 5 sec to 1 min [40 ]. The images were then normalized using the method previously reported by us [26 (link)].