Goat anti rabbit igg hrp

Manufactured by GeneTex

Sourced in United States

Goat anti-rabbit IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and quantify rabbit primary antibodies in various immunoassays and immunodetection techniques.

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Lab products found in correlation

Goat anti mouse igg hrp, by GeneTex (2 mentions) Mouse anti ocn, by Abcam (1 mentions) Goat anti mouse igg hrp, by Thermo Fisher Scientific (1 mentions) Rabbit anti bsp, by Abcam (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Osteosoft, by Merck Group (1 mentions) Amersham hybond p 0, by GE Healthcare (1 mentions) Imagequant las 4000 system, by GE Healthcare (1 mentions) Sig 39220, by BioLegend (1 mentions) Sig 39320, by BioLegend (1 mentions) Gtx213110 01, by GeneTex (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Gapdh antibody, by Proteintech (1 mentions) Rabbit anti kras g12d, by Cell Signaling Technology (1 mentions) Rabbit anti kras, by Cell Signaling Technology (1 mentions) Immobilon forte western hrp substrate, by Merck Group (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Sds page electrophoresis, by Bio-Rad (1 mentions) Vegfr 2, by Novus Biologicals (1 mentions) Biospectrum 500 imaging system, by Analytik Jena (1 mentions)

4 protocols using goat anti rabbit igg hrp

Histological Analysis of Rat Calvarial Bone

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After μCT scanning at week 8, we sacrificed the rats and removed the calvarial bones. The bone specimens were fixed in 4% aqueous phosphate formaldehyde for 3 days at room temperature and subsequently decalcified by immersing in Osteosoft^® (Merck) for 14 days. Decalcified specimens were washed in PBS before undergoing dehydration, paraffin embedding, and sagittal slicing into 10-μm-thick sections. Sections were then rehydrated and stained with H&E.
Alternatively, rehydrated sections were subjected to trypsin treatment for 1 h at 37°C for antigen retrieval, followed by blocking in 5% skimmed milk and immunohistochemical staining. The primary antibodies were rabbit anti-BSP (1:200, Abcam) and mouse anti-OCN (1:200, Abcam). The secondary antibodies were goat anti-rabbit IgG-HRP (1:5000, GeneTex) and goat anti-mouse IgG-HRP (1:5000, Invitrogen). The sections were developed with hydrogen peroxide and 3,3′‐diaminobenzidine (Sigma) and counterstained with Eosin for visualization.

Truong V.A., Hsu M.N., Kieu Nguyen N.T., Lin M.W., Shen C.C., Lin C.Y, & Hu Y.C. (2019). CRISPRai for simultaneous gene activation and inhibition to promote stem cell chondrogenesis and calvarial bone regeneration. Nucleic Acids Research, 47(13), e74.

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Aβ Assembly Modulation by TDP-43 in APP/PS1ΔE9 Mice

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Western blot was performed to observe the changes in Aβ assembly after injecting TDP-43 to the APP/PS1ΔE9 mice. The concentration of the extracellular-enriched fraction and Triton-soluble fraction were determined and adjusted to the same protein concentration (48 μg in 20 μL). The samples were mixed with 6x loading buffer and heated in 95 °C for 10 min, then loaded into a 4–15% Tris-glycine gel and resolved at 100 V for 120 min. The separated proteins were transferred to a PVDF membrane (Amersham Hybond P 0.45, GE healthcare, Chicago, Illinois, USA) and blocked with 5% non-fat milk. The mouse monoclonal 6E10 antibody (1:5000, SIG-39320, BioLegend, San Diego, CA, USA) and mouse monoclonal 4G8 antibody (1:5000, SIG-39220, BioLegend, San Diego, CA, USA) were mixed and used for Aβ detection. A mouse monoclonal GAPDH antibody (1:5000, Proteintech, Rosemont, IL, USA) was used for loading control. The secondary antibody used for 6E10/4G8 mixture is goat anti-mouse IgG-HRP (GTX213111-01, Genetex, CA, USA) and for GAPDH is goat anti-rabbit IgG-HRP (GTX213110-01, Genetex, CA, USA). Immobilon Western Chemiluminescent HRP substrate (Merck, Darmstadt, Germany) was used for developing. The detection was carried out with Imagequant LAS4000 system (GE Healthcare, Life sciences, Hungary) and analyzed using ImageJ (NIH, Bethesda, MD, USA).

Shih Y.H., Tu L.H., Chang T.Y., Ganesan K., Chang W.W., Chang P.S., Fang Y.S., Lin Y.T., Jin L.W, & Chen Y.R. (2020). TDP-43 interacts with amyloid-β, inhibits fibrillization, and worsens pathology in a model of Alzheimer’s disease. Nature Communications, 11, 5950.

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Western Blot Analysis of Protein Extracts

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Cells were sonicated or homogenized in RIPA buffer (20 mM Tris‐HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% NP‐40, 0.1% SDS, and 1% sodium deoxycholate). After being clarified by centrifugation at 12 000 g for 15 min at 4 °C, the supernatant was collected for Bradford protein assay. Western blotting was performed as previously reported.^[⁵⁸ (link)
^] Briefly, equal molarity of protein extracts was loaded and separated in an SDS–PAGE, and transferred to a PVDF membrane. After being blocked with 5% skimmed milk at RT for 1 h, the membrane was incubated with primary antibodies (rabbit anti‐MUC4 (1:1000; Thermo Fisher Scientific #35‐4900), ribbit anti‐GFP (1:10 000; GeneTex #GTX113617), rabbit anti‐KRAS G12D (1:1000; Cell Signaling Technology #14 429), rabbit anti‐KRAS (1:2000; Cell Signaling Technology #67 648), rabbit anti‐Activin A (1:1000; GeneTex #GTX108405), mouse anti‐GAPDH (1:10 000; GeneTex #GTX627408) at 4 ˚C overnight and treated with Goat Anti‐Rabbit IgG (HRP) (1:3000; GeneTex #GTX213110‐01) and Goat Anti‐Mouse IgG (HRP) (1:3000; GeneTex #GTX213111‐01) antibodies at room temperature for 1 h. Chemiluminescent detection of the horseradish peroxidase reaction was performed using Immobilon Forte Western HRP substrate (Merck #WBLUF0500) according to the manufacturer's instruction and filmed by ChemiDoc MP Imaging System (Biorad).

Hu C., Huang C., Hsu M., Chien H., Wu P., Chen Y., Jeng Y., Tang S., Chung M., Shen C., Chang M., Chang Y., Tien Y, & Lee W. (2023). Oncogenic KRAS, Mucin 4, and Activin A‐Mediated Fibroblast Activation Cooperate for PanIN Initiation. Advanced Science, 10(36), 2301240.

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ARPE-19 Cells Treated with G570 and Blue Light

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ARPE-19 cells were seeded at a density of 4 × 10⁵ in 6 cm dishes. After 24 h, cells were treated with 0.5 µM G570 and 150 lux blue light. After 24 h, cells were harvested in RIPA lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EGTA, and 1% w/v Nonidet P-40) and placed on ice. Collected cells were centrifuged at 14,000 g for 10 min. Total protein was collected, and the concentration was measured using Bradford reagent (Bio-Rad Laboratories, Hercules, CA, USA). Total proteins were isolated by SDS-PAGE electrophoresis (Bio-Rad Laboratories, CA, USA) and transferred onto PVDF membranes (Millipore Sigma, Burlington, MA, USA). After blocking with 5% w/v BSA (Sigma-Aldrich, Dublin, Ireland) for 40 min, the membranes were incubated with primary antibodies against 1:500 VEGFA-189 (Novus Biologicals, Centennial, CO, USA), 1:500 VEGFR2 (Novus Biologicals, CO, USA), 1:1000 ERK (Cell Signaling, Danvers, MA, USA), 1:1000 FAK (Abcam, Cambridge, UK), 1:1000 p38 (Cell Signaling, MA, USA), 1:2000 Akt (Cell Signaling, MA, USA), and 1:5000 eNOS (BD, Franklin Lakes, NJ, USA). Then, the membranes were washed twice with TBST and probed with 1:5000 goat anti-rabbit IgG (HRP) or 1:5000 goat anti-mouse IgG (HRP) (GeneTex, Irvine, CA, USA). The expression of these proteins was detected with HRP substrate luminol reagent by a BioSpectrum^® 500 Imaging System (UVP, Upland, CA, USA).

Hsu T.J., Nepali K., Tsai C.H., Imtiyaz Z., Lin F.L., Hsiao G., Lai M.J, & Cheng Y.W. (2021). The HDAC/HSP90 Inhibitor G570 Attenuated Blue Light-Induced Cell Migration in RPE Cells and Neovascularization in Mice through Decreased VEGF Production. Molecules, 26(14), 4359.

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