Anti omp

Manufactured by Fujifilm

Anti-OMP is a laboratory equipment product manufactured by Fujifilm. It is designed to detect and quantify Outer Membrane Proteins (OMPs) in various biological samples. The product utilizes advanced analytical techniques to provide accurate and reliable measurements of OMP levels, which are important indicators in various research and diagnostic applications.

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Lab products found in correlation

Nitrocellulose membrane, by Cytiva (1 mentions) Chemidoc eq system, by Bio-Rad (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Leica speii, by Leica camera (1 mentions) Hoechst, by Merck Group (1 mentions) Alexa fluor 488 or 594 conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Cm3050, by Leica camera (1 mentions) Anti pcna, by Merck Group (1 mentions) Anti sox2, by R&D Systems (1 mentions) Anti gαo, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 555 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti goat, by Thermo Fisher Scientific (1 mentions) Xylazine, by Bayer (1 mentions) Ketamine, by Bayer (1 mentions) Anti gap 43, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)

4 protocols using anti omp

Immunoblotting of Octopus Olfactory Proteins

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2 µl (200 ng/μl) of total protein extract from O. vulgaris tissues respectively: olfactory epithelium (OE), supra- and suboesophageal masses, optic lobes and arm were applied on nitrocellulose membrane (Whatman) and let dry at room temperature. After 1 h incubation with non-fat milk 5% blocking solution, we incubated with anti-OMP (dilution 1:500; Wako; #019-2229 RRID: AB_664696) overnight at 4°C. The membrane was then washed extensively using Tris buffer with Tween 20 (Sigma Aldrich) 0.05% (TBS-T) and subsequently incubated with a rabbit anti goat horseradish peroxidase conjugated (dilution 1:5000; Thermo Fisher Scientific) for 1 h. After several rinses with TBS-T, immunopositive dots were visualized using the SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology) in accordance with the manufacturer's instructions using a Chemidoc EQ System (Bio-Rad).
Anti-PCNA specificity has been determined in a recent study on octopus arm regeneration (Fossati et al., 2013 (link)) and supported by loss of labelling in controls with the antibody pre-adsorbed with its antigen. Furthermore, using CLUSTALW2 database, we performed an alignment of mouse PCNA whole protein sequence with PCNA protein sequence annotated in Octopus bimaculoides genome recently published (Albertin et al., 2015 (link)).

Polese G., Bertapelle C, & Di Cosmo A. (2016). Olfactory organ of Octopus vulgaris: morphology, plasticity, turnover and sensory characterization. Biology Open, 5(5), 611-619.

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Immunohistochemical Analysis of Olfactory Markers

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VNOs embedded in OCT medium were sliced in 16 μm thick sections in a cryostat apparatus (Leica CM 3050S). Slices were incubated in blocking solution containing 0.5% Triton X-100 and 5% normal horse serum in 0.1 M TBS for 2 h at room temperature (RT). Primary antibody incubation was performed overnight (o/n) at 4 °C with anti-OMP (Wako goat polyclonal; 1:2000), anti-PCNA (Sigma, rabbit monoclonal; 1:2000), and anti-Sox2 (R&D Systems, goat polyclonal; 1:300). For PCNA immunostaining, slices were incubated in 10 mM citrate buffer (100 °C; pH, 6.0) for 5 min prior staining. Sections were incubated with Alexa Fluor 488- or 594-conjugated secondary antibodies (Jackson Laboratories, 1:500) for 2 h at RT. Hoechst (Sigma, 1:10,000) was added during 5 min after the secondary antibody incubation for nuclei visualization. Imaging was performed using a vertical confocal microscope Leica SPEII. Final images were assembled in Adobe Illustrator.

Portalés A., Chamero P, & Jurado S. (2023). Natural and Pathological Aging Distinctively Impacts the Pheromone Detection System and Social Behavior. Molecular Neurobiology, 60(8), 4641-4658.

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Immunofluorescence Analysis of Olfactory Markers

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Mice were anesthetized with a 4:1 cocktail of ketamine and xylazine (Bayer) and perfused transcardially with 0.9% saline solution followed by 0.1 M phosphate buffer (PB) containing 4% paraformaldehyde. The brains were removed, postfixed for 6 h in 4% paraformaldehyde, and incubated overnight in 0.1 M PB containing 30% sucrose. Cryosections (30 μm thick) were mounted on SuperFrost Plus glass slides for immunofluorescence analysis. Tissue sections were washed (10 min) in PBS, incubated in blocking solution containing 0.5% Triton X-100, 4% horse serum, and PBS (1 h, room temperature), and incubated overnight at 4°C in blocking solution containing the first primary antibody. The tissue was then washed in PBS (10 min), followed by incubation in secondary antibody for 1 h at room temperature. Primary antibodies used were anti-Gαo (1:200, rabbit polyclonal; Santa Cruz Biotechnology) and anti-OMP (1:5000, goat polyclonal, Wako Chemicals). Secondary antibodies used were Alexa-Fluor 488 donkey anti-goat and Alexa fluor 555 donkey anti-rabbit (1:1000; Invitrogen).

Oboti L., Pérez-Gómez A., Keller M., Jacobi E., Birnbaumer L., Leinders-Zufall T., Zufall F, & Chamero P. (2014). A wide range of pheromone-stimulated sexual and reproductive behaviors in female mice depend on G protein Gαo. BMC Biology, 12, 31.

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Immunofluorescence Staining of Paraffin and Frozen Tissue

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Paraffin-embedded sections were deparaffinized and autoclaved at 121°C for 20 min in Target Retrieval Solution (Dako Japan Inc., S1700). Frozen sections were briefly washed in PBS and incubated with 0.5 % SDS (v/v) in PBS for 15 min to retrieve antigen. Blocking was performed with 2 % bovine serum albumin (BSA) in PBS with 0.1 % Triton X-100 for 10 min for the paraffin sections and with 5 % BSA in PBS with 0.3 % Triton for the frozen sections before overnight incubation with the following primary antibodies at 4°C in a humidified chamber: anti-OMP (goat polyclonal, 1:3,000; Wako Chemicals, 544-10001-WAKO, RRID: AB_664696), anti-GAP43 (chicken polyclonal, 1:250; Thermo Fisher Scientific, PA5-95660, RRID: AB_2807462), anti-activated caspase-3 (rabbit polyclonal, 1:500; Cell Signaling Technology Inc., 9661, RRID: AB_2341188), anti-Ki67 (rabbit monoclonal, 1:300; Lab Vision, RM-9106-S1, RRID: AB_149792), and anti-c-Fos (rabbit IgG, 1:1,000; Santa Cruz Biotechnology Inc., 2250, RRID: AB_2247211). After the overnight incubation, tissues were washed with PBS and were incubated with the following secondary antibodies for 1 h at room temperature: donkey anti-goat Alexa Fluor 488 (1:100; Invitrogen, A32814, RRID: AB_2762838), donkey anti-chicken Alexa Fluor 488 (1:250; Jackson ImmunoResearch

Kuboki A., Kikuta S., Otori N., Kojima H., Matsumoto I., Reisert J., & Yamasoba T. (2021). Insulin-dependent maturation of newly generated olfactory sensory neurons after injury.

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