Imap fp screening express kit

LPS (Escherichia coli serotype) and PIM-447 were purchased from Sigma (St. Louis, Mo, USA) and Selleck Chemicals (Houston, TX, USA), respectively. Recombinant PIM-1, PIM-2, and PIM-3 kinases were purchased from Life Technologies (Carlsbad, CA, USA), Abcam (Cambridge, UK), and Millipore (Burlington, MA, USA), respectively. The IMAP™ FP Screening Express Kit was obtained from Molecular Devices (San Jose, CA, USA). The 5-FAM-labelled Bad peptide (5-FAM-RSRHSSYPAGT) was purchased from AnaSpec (Fremont, CA, USA). Dithiothreitol was obtained from Sigma. The primary antibodies against TLR4, p-IKK α/β, p-NFκB/P65, iNOS, MyD88, ASC, caspase-1, and NLRP3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-IL-1β antibody was purchased from Novus Biologicals (Centennial, CO, USA). Anti-p-Bad and anti-PIM-1 antibodies were purchased from Abcam (Cambridge, UK). Anti-β-actin antibody was purchased from Sigma-Aldrich (St. Louise, MO, USA). Anti-horse anti-mouse IgG-horseradish peroxidase (HRP) and anti-goat anti-rabbit IgG-HRP antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). PIM-1 siRNA was purchased from Life Technologies (Carlsbad, CA, USA).