Tissue samples were homogenized in lysis buffer [20 mM Tris (pH 7.4), 150 mM NaCl, 10 mM EDTA and 1% Triton X-100 with a complete protease inhibitor cocktail (Roche)]. TNFα and IL6 levels in intestinal cell extracts were measured by the Mouse TNF-α Quantikine HS ELISA Kit (MHSTA50, R&D Systems) and Mouse IL-6 Quantikine ELISA Kit (M6000B, R&D Systems), according to the manufacturer's protocols. The results were analyzed using a Synergy H4 Reader (BioTek Instruments).
Mouse tnf α quantikine hs elisa kit
Manufactured by R&D Systems
Sourced in United States
The Mouse TNF-α Quantikine HS ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure mouse tumor necrosis factor alpha (TNF-α) levels in cell culture supernates, serum, and plasma.
Automatically generated - may contain errors
3 protocols using mouse tnf α quantikine hs elisa kit
1
Quantifying Inflammatory Cytokines in Intestinal Cells
2
Measuring Inflammatory Cytokines
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IL-1β in supernatants and pro-IL-1β in cell lysates were measured using the Human IL-1β/IL-1F2 DuoSet ELISA Development System (R&D Systems). TNF (tumor necrosis factor)-α was measured using the Human TNFα DuoSet ELSA Development System (R&D Systems). Mice serum IL-1β and TNFα were measured using the Mouse IL-1β/IL-F2 Quantikine HS ELISA Kit (R&D Systems) and the Mouse TNFα Quantikine HS ELISA Kit (R&D Systems). Human plasma IL-18 was detected using the Human Total IL-18 DuoSet ELSA Development System (R&D Systems).
3
Serum Biomarkers in Liver Disease
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The blood samples collected from the BDL or sham groups were incubated at room temperature for 2 h. To collect the serum, the blood samples were centrifuged at 2000g for 20 min. The serum level of TNFα was determined using the Mouse TNFα Quantikine HS ELISA kit (R&D systems, Minneapolis, MN, USA). The serum levels of non-esterified fatty acids (NEFA) were determined by an enzymatic colorimetric method (LabAssayTM NEFA, Wako, Tokyo, Japan).
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