Dfc365fx monochrome digital camera

Manufactured by Leica camera

The DFC365FX is a monochrome digital camera designed for laboratory and scientific applications. It features a high-resolution, low-noise sensor that captures detailed images. The camera is capable of capturing high-quality monochrome images for a variety of research and analysis purposes.

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Lab products found in correlation

Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions) Las x acquisition software, by Leica camera (1 mentions) Axiocam, by Zeiss (1 mentions) Coolled, by Zeiss (1 mentions) Phospho histone h2ax, by Merck Group (1 mentions) Mowiol 4 88, by Merck Group (1 mentions) Cenpf, by Abcam (1 mentions) Spe single channel confocal laser scanning microscope, by Leica camera (1 mentions) Prime 95b scmos camera, by Leica camera (1 mentions) Rad51, by Abcam (1 mentions) Las af software, by Leica camera (1 mentions) 8 well chamber culture slides, by BD (1 mentions) Anti human cd3 clone okt3, by Thermo Fisher Scientific (1 mentions) Poly l lysine solution, by Merck Group (1 mentions) Vectashield mounting medium with dapi, by Vector Laboratories (1 mentions)

3 protocols using dfc365fx monochrome digital camera

Multimodal Microscopy Imaging Techniques

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Images were acquired by a Leica STED high-resolution laser scanning confocal microscope (Leica, Wetzlar, Germany) and a 20 oil/1.4 NA objective. Quantification of extent of colocalization was carried out by Imaris software (Bitplane, Zurich, Switzerland).
For histology of rats used in acute silencing experiments, images were acquired by Zeiss Axisoskop2 with pE2 LED excitation system (CoolLED, UK) and analyzed using a Zeiss Axiocam and AxioVision software. For tissue from acute optogenetic silencing experiments, images were acquired using a Leica DMI6000 inverted epifluorescence microscope equipped with Leica DFC365FX monochrome digital camera and Leica LAS-X acquisition software.

Hayat H., Regev N., Matosevich N., Sales A., Paredes-Rodriguez E., Krom A.J., Bergman L., Li Y., Lavigne M., Kremer E.J., Yizhar O., Pickering A.E, & Nir Y. (2020). Locus coeruleus norepinephrine activity mediates sensory-evoked awakenings from sleep. Science Advances, 6(15), eaaz4232.

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Immunofluorescence Staining of DNA Damage Markers

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Cells grown on glass coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton-X 100. To prevent non-specific antibody binding, slides were blocked with 1% BSA. Cells were incubated with primary antibody at room temperature for one hour (phospho-histone H2A.X (1:10000, Millipore #05–636); RAD51(1:1000, Abcam); CENPF (1:500, Abcam #5) diluted in 1% BSA. After incubation with secondary antibody (Molecular Probes, Invitrogen, UK) the nuclei were stained 100 ng/µL DAPI. Coverslips were mounted with Mowiol 4–88 (Sigma-Aldrich, UK). Cells were imaged with a Leica DMI6000 inverted epifluorescence microscope with 100x lens and Photometrics Prime 95B sCMOS camera or a Leica SPE single channel confocal laser scanning microscope with 40x lens and Leica DFC365FX monochrome digital camera.

Parker C., Chambers A.C., Flanagan D.J., Ho J.W., Collard T.J., Ngo G., Baird D.M., Timms P., Morgan R.G., Sansom O.J, & Williams A.C. (2022). BCL-3 loss sensitises colorectal cancer cells to DNA damage by targeting homologous recombination. DNA Repair, 115, 103331.

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Quantifying T-Cell Actin Cytoskeleton

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8-well chamber culture slides (BD Falcon, San Jose, CA, USA) were coated with 0.01% poly-L-lysine solution (Sigma-Aldrich) for 15 minutes at room temperature and subsequently with 10µg/ml anti-human CD3 (clone OKT3; eBioscience) overnight at 4°C. 0.5×10 6 (for 1 minute of spreading) or 0.2×10 6 (for 3 and 5 minute of spreading) cells were plated gently onto the slides and allowed to spread for 1, 3 or 5 minutes. Cells were fixed with 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes and blocked with 5% goat serum in 10% FBS/0.02% sodium azide in PBS for an hour. Cells were then stained with rhodamine phalloidin (Thermo Fisher Scientific)
according to the manufacturer's instructions. After washing, the cell specimens were mounted with Vectashield Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA, USA). Slides were analyzed using Leica DM4500B fluorescence microscope (Leica Microsystems, Wetzlar, Germany) with a 100× oil objective. Images were acquired using a Leica DFC365 FX monochrome digital camera and Leica LAS AF software. Percentage of spreading was calculated by counting at least 100 cells per experimental condition. Cells that form an F-actin ring at the cell periphery were scored as positive while cells without an Factin ring were scored as negative.

Lim S.P., Ioannou N., Ramsay A.G., Darling D., Gäken J, & Mufti G.J. (2018). miR-181c-BRK1 axis plays a key role in actin cytoskeleton-dependent T cell function. Journal of leukocyte biology, 103(5).

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