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2 protocols using hexane

1

Standard Analytical Reagent Procurement

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Analytical grade acetone, acetic acid, anhydrous sodium acetate, ethanol, hexane, hydrochloric acid, potassium chloride, sodium carbonate, and sulfuric acid were obtained from Chem Supply (Port Adelaide, South Australia, Australia). High pressure liquid chromatography (HPLC) grade methanol, acetonitrile, formic acid, and ammonium acetate as well as analytical standards (Folin–Ciocalteu reagent, vanillin, gallic acid, cyanidin-3-O-glucoside, (+)-catechin, coumestrol, formononetin, genistein, and naringenin) were purchased from Sigma Aldrich (Australia).
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2

Graphite Powder Production and Functionalization

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Natural graphite rocks
were obtained from a local mining site (Uley, Eyre Peninsula, South
Australia, Australia), crushed into powder using a benchtop ring mill
(Rocklabs), and sifted using a 25 μm sieve. Potassium permanganate
(Sigma-Aldrich), 85% w/w phosphoric acid (Chem-Supply), 98% sulfuric
acid (Chem-Supply), 30% hydrogen peroxide (Chem-Supply), 36% hydrochloric
acid (Chem-Supply), 64–65% hydrazine monohydrate (Sigma-Aldrich),
30% ammonia (Chem-Supply), cysteamine hydrochloride (Sigma-Aldrich), N,N-dimethylforamide (DMF, Chem-Supply),
2,2-azobis-2-methylpropionitrile (AIBN, Sigma-Aldrich), hexane (Chem-Supply),
ethyl acetate (Chem-Supply), and ethanol (Chem-Supply) were used directly
without prior purification.
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