Cell morphology was investigated either under the light microscope (Leica MC170 HD) or a fluorescence microscope (Nikon). The viability of cells was analyzed with the LIVE/DEAD BacLight fluorescence stain from Life Technologies (Thermo Fisher Scientific). For all measurements, live cells were mounted onto 1% agarose pads supplemented with TB medium prior to microscopy analysis. Phase-contrast and fluorescence images were captured using a Ti eclipse inverted research microscope (Nikon) with a ×100/1.45 numerical aperture (NA) objective (Nikon). Image processing and cell length measurement was conducted by Fiji ImageJ 1.8.084 (link).
Mc170 hd
Manufactured by Nikon
The MC170 HD is a Nikon microscope camera designed for laboratory applications. It captures high-definition images and video with a 5.0-megapixel CMOS sensor. The camera connects to microscopes via a C-mount interface and provides live image previewing on a computer through a USB 3.0 connection.
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Lab products found in correlation
3 protocols using mc170 hd
1
Cell Morphology and Viability Microscopy
2
Cell Morphology and Viability Microscopy
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Cell morphology was investigated either under the light microscope (Leica MC170 HD) or a fluorescence microscope (Nikon). Viability of cells was analyzed with the LIVE/DEAD BacLight fluorescence stain from Life Technologies (Thermo Fisher Scientific). For all measurements, live cells were mounted onto 1% agarose pads supplemented with TB medium prior to microscopy analysis.
Phase-contrast and fluorescence images were captured using a Ti eclipse inverted research microscope (Nikon) with a 100x/1.45 numerical aperture (NA) objective (Nikon). Image processing and cell length measurement was conducted by Fiji ImageJ 1.8.0 (75) .
Phase-contrast and fluorescence images were captured using a Ti eclipse inverted research microscope (Nikon) with a 100x/1.45 numerical aperture (NA) objective (Nikon). Image processing and cell length measurement was conducted by Fiji ImageJ 1.8.0 (75) .
3
Morphological Analysis of SP Coatings
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After dip-coating, morphological analysis of the SPs coating has been carried out by optical microscopy and scanning electron microscopy (SEM). Optical micrographs are acquired by a digital camera (Leica MC170 HD), using a Nikon optical microscope in reflection mode (objective magnifications ×50 and ×1000). Optical microscopy allows to determine the total surface coverage, and the surface covered by either SPs single layer (SL), SPs multi-layer (ML) or non-assembled SPs. This analysis has been carried out at multiple areas along the coated surface (see Figure S-2). The confirmation of each type of coating is demonstrated by SEM. SEM of SPs coatings is performed with a Hitachi S4700 at following operating parameters: 5 kV and 7.4 mm WD. To improve the sample conductivity, an 80-100 Å thin layer of Au is sputtered onto the SPs coated surface.
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