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Phencyclidine pcp

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Phencyclidine (PCP) is a chemical compound used in research laboratories for various scientific applications. It is a dissociative anesthetic agent that can induce altered perceptions, feelings, and consciousness. The core function of PCP is to serve as a research tool for studies in fields such as neuroscience, pharmacology, and psychology. Its use is strictly controlled and regulated due to the potential for abuse and adverse effects.

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Lab products found in correlation

S ketamine, by Merck Group (2 mentions) R ketamine, by Cayman Chemical (2 mentions) Mk 801, by Merck Group (2 mentions) Desipramine, by Merck Group (2 mentions) Clozapine, by Merck Group (1 mentions) Haloperidol, by Merck Group (1 mentions) Haloperidol, by Bio-Techne (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Clozapine, by Bio-Techne (1 mentions) Kainic acid, by Hello Bio (1 mentions) U73122, by Abcam (1 mentions) Tween 80, by Merck Group (1 mentions) Vu0360172, by Bio-Techne (1 mentions)

6 protocols using phencyclidine pcp

1

Pharmacological Reagent Preparation

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Phencyclidine (PCP), haloperidol, and clozapine were purchased from Sigma-Aldrich (St. Louis, MO). PCP was dissolved in sterile water (Butler-Schein Animal Health, Dublin, OH); haloperidol (Bio-Techne, Minneapolis, MN) was dissolved in sterile-filtered DMSO (Sigma-Aldrich, St. Louis, MO) and diluted in sterile water (Hospira Inc., Lake Forest, IL). For haloperidol, the final concentration of DMSO did not exceed 0.05% of the injected volume. clozapine (Bio-Techne) was dissolved in 0.2% glacial acetic acid with sterile water. The glacial acetic acid did not exceed 0.01% the final injected volume.
Monroe T.O., Garrett M.E., Kousi M., Rodriguiz R.M., Moon S., Bai Y., Brodar S.C., Soldano K.L., Savage J., Hansen T.F., Muzny D.M., Gibbs R.A., Barak L., Sullivan P.F., Ashley-Koch A.E., Sawa A., Wetsel W.C., Werge T, & Katsanis N. (2020). PCM1 is necessary for focal ciliary integrity and is a candidate for severe schizophrenia. Nature Communications, 11, 5903.
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2

Synthesis and Characterization of Ketamine Analogues

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(R,S)-ketamine, (S)-ketamine, desipramine, MK-801, phencyclidine (PCP) (Sigma-Aldrich, St. Louis, MO, USA), (R)-ketamine (Cayman Chemicals, Ann Arbor, MI, USA) and NBQX (National Institute of Mental Health Chemical Synthesis and Drug Supply Program) were dissolved in 0.9% saline. (2S,6S)-HNK, (2R,6R)-HNK, and 6,6-dideuteroketamine hydrochloride were synthesised and characterised both internally at the National Center for Advancing Translational Sciences and at SRI International (Menlo Park, CA, USA) as described in Supplementary Information. Absolute and relative stereochemistry for (2S,6S)-HNK and (2R,6R)-HNK were confirmed by small molecule x-ray crystallography, as described in the Supplementary Information.
All drugs were dissolved in 0.9% saline, and administered intraperitoneally (i.p.) in a volume of 7.5 ml/kg of body mass by a male experimenter for the behavioural studies. Corticosterone (4-pregnen-11β, 21-diol-3, 20-dione 21-hemisuccinate; Steraloids, Newport, RI, USA) was dissolved in tap water. For the electrophysiology recordings, test drugs were diluted in artificial cerebrospinal fluid (ACSF).
Zanos P., Moaddel R., Morris P.J., Georgiou P., Fischell J., Elmer G.I., Alkondon M., Yuan P., Pribut H.J., Singh N.S., Dossou K.S., Fang Y., Huang X.P., Mayo C.L., Wainer I.W., Albuquerque E.X., Thompson S.M., Thomas C.J., Zarate CA J.r, & Gould T.D. (2016). NMDAR inhibition-independent antidepressant actions of ketamine metabolites. Nature, 533(7604), 481-486.
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3

PCP and Receptor Modulators Protocol

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Phencyclidine (PCP; Sigma-Aldrich, Gillingham, UK; dissolved in 0.9% saline solution); VU0360172 (N-cyclobutyl-6-((3-fluorophenyl)ethynyl)picolin-amide; Tocris (Bio-Techne), Pittsburgh, USA; stock 10 mM in DMSO); VU0409551(4-fluorophenyl)(2-(phenoxymethyl)-6,7-dihydrooxazolo[5,4-c]pyridin-5(4H)-yl)methanone; Tocris (Bio-Techne), Pittsburgh, USA; stock 10 mM in DMSO); Kainic acid (Hello Bio, Bristol, UK; stock 0.25 mM, in dH2O); Carbachol (Carbamoylcholine chloride; Sigma-Aldrich, Gillingham, UK; stock 50 mM in dH2O); MTEP (3-((2-Methyl-4-thiazolyl)ethynyl)pyridine; Hello Bio, Bristol, UK; stock 200 µM in DMSO); Go6983 (Hello Bio, Bristol, UK; stock 10 µM in DMSO); U73122 (Abcam, Cambridge, UK; stock 10 mM in DMSO).
Brown J., Grayson B., Neill J.C., Harte M., Wall M.J, & Ngomba R.T. (2023). Oscillatory Deficits in the Sub-Chronic PCP Rat Model for Schizophrenia Are Reversed by mGlu5 Receptor-Positive Allosteric Modulators VU0409551 and VU0360172. Cells, 12(6), 919.
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4

Synthesis and Characterization of Ketamine Analogues

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(R,S)-ketamine, (S)-ketamine, desipramine, MK-801, phencyclidine (PCP) (Sigma-Aldrich, St. Louis, MO, USA), (R)-ketamine (Cayman Chemicals, Ann Arbor, MI, USA) and NBQX (National Institute of Mental Health Chemical Synthesis and Drug Supply Program) were dissolved in 0.9% saline. (2S,6S)-HNK, (2R,6R)-HNK, and 6,6-dideuteroketamine hydrochloride were synthesised and characterised both internally at the National Center for Advancing Translational Sciences and at SRI International (Menlo Park, CA, USA) as described in Supplementary Information. Absolute and relative stereochemistry for (2S,6S)-HNK and (2R,6R)-HNK were confirmed by small molecule x-ray crystallography, as described in the Supplementary Information.
All drugs were dissolved in 0.9% saline, and administered intraperitoneally (i.p.) in a volume of 7.5 ml/kg of body mass by a male experimenter for the behavioural studies. Corticosterone (4-pregnen-11β, 21-diol-3, 20-dione 21-hemisuccinate; Steraloids, Newport, RI, USA) was dissolved in tap water. For the electrophysiology recordings, test drugs were diluted in artificial cerebrospinal fluid (ACSF).
Zanos P., Moaddel R., Morris P.J., Georgiou P., Fischell J., Elmer G.I., Alkondon M., Yuan P., Pribut H.J., Singh N.S., Dossou K.S., Fang Y., Huang X.P., Mayo C.L., Wainer I.W., Albuquerque E.X., Thompson S.M., Thomas C.J., Zarate CA J.r, & Gould T.D. (2016). NMDAR inhibition-independent antidepressant actions of ketamine metabolites. Nature, 533(7604), 481-486.
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5

Dosing and Administration of Psychoactive Compounds

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SAR502250 (Sanofi Medicinal Chemistry), amphetamine, fluoxetine, lithium chloride, phencyclidine (PCP) (Sigma-Aldrich, Saint-Quentin Fallavier, France) were dissolved or suspended in distilled water with 0.6% methylcellulose and the addition of 5% Tween 80 (Sigma-Aldrich) or 2% Cremophor in in vivo studies and suspended in dimethylsulfoxyde (DMSO) at 10 mM in in vitro experiments. Doses refer to the weight of the free base. SAR502250 was administered orally (per os, p.o.) in the behavioral tests with the exception of the chronic mild stress procedure where it was administered intraperitoneally (i.p.). This administration route was chosen because exploratory experiments showed that it is more suitable in this test than the p.o. route. Different treatment schedules were chosen because some of the procedures used required repeated administration to observe a drug effect (e.g. chronic mild stress). The amount of vehicle was adjusted to be the same for all the doses and controls. Volume of administration was 10 or 20 ml/kg in mice, 1 or 5 ml/kg in rats. All drug solutions were prepared fresh daily.
Griebel G., Stemmelin J., Lopez-Grancha M., Boulay D., Boquet G., Slowinski F., Pichat P., Beeské S., Tanaka S., Mori A., Fujimura M, & Eguchi J. (2019). The selective GSK3 inhibitor, SAR502250, displays neuroprotective activity and attenuates behavioral impairments in models of neuropsychiatric symptoms of Alzheimer’s disease in rodents. Scientific Reports, 9, 18045.
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6

Comparative Pharmacology of PCP Derivatives

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Phencyclidine (PCP; Sigma, UK); VU0360172 (N-cyclobutyl-6-((3fluorophenyl)ethynyl)picolin-amide; Tocris/Bio-Techne); VU0409551 (4-fluorophenyl)(2-(phenoxymethyl)-6,7-dihydrooxazolo [5,4-c] pyridin-5(4H)-yl)methanone; Tocris/Bio-Techne)
Brown J., Iacovelli L., Di Cicco G., Grayson B., Rimmer L., Fletcher J., Neill J.C., Wall M.J., Ngomba R.T, & Harte M. (2022). The comparative effects of mGlu5 receptor positive allosteric modulators VU0409551 and VU0360172 on cognitive deficits and signalling in the sub-chronic PCP rat model for schizophrenia. Neuropharmacology, 208.
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