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Orca flash4.0 hamamatsu digital camera c11440

Manufactured by Hamamatsu Photonics
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Sourced in Japan

The ORCA-Flash4.0 HAMAMATSU digital camera C11440 is a scientific-grade, back-illuminated sCMOS camera. It features a high quantum efficiency, low readout noise, and fast frame rates. The camera is designed for a wide range of applications requiring high-performance imaging, such as fluorescence microscopy and scientific research.

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Lab products found in correlation

Metamorph software, by Molecular Devices (4 mentions) Adipored assay reagent, by Lonza (2 mentions)

4 protocols using orca flash4.0 hamamatsu digital camera c11440

1

Measuring Mature Adipocyte Size

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For mature adipocyte size measurement, coverslips with adipocytes attached were transferred to a bath chamber on the stage of an inverted microscope (IX73, Olympus) and visualized using a 20x or 40x objective lens (Olympus). Cell images were captured using an ORCA-Flash4.0 HAMAMATSU digital camera C11440 (HAMAMATSU, Japan) and recorded using Metamorph software (Molecular Devices, Inc.). The cross-sectional area was measured as an indicator of the cell size, using Metamorph software.
Zhang Y., Xie L., Gunasekar S.K., Tong D., Mishra A., Gibson W.J., Wang C., Fidler T., Marthaler B., Klingelhutz A., Abel E.D., Samuel I., Smith J.K., Cao L, & Sah R. (2017). SWELL1 is a regulator of adipocyte size, insulin signaling and glucose homeostasis. Nature cell biology, 19(5), 504-517.
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2

Lipid Quantification by AdipoRed Assay

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Lipid content was quantified using AdipoRed assay reagent (Lonza). In brief, differentiated cells were washed with PBS (pH7.4) three times, and incubated with AdipoRed solution at room temperature for 10 minutes. Cells were again washed gently with PBS. Fluorescence signal was measured with an excitation wavelength of 485 nm and an emission wavelength of 572 nm. Images were captured using an ORCA-Flash4.0 HAMAMATSU digital camera C11440 (HAMAMATSU, Japan). Fluorescence intensity quantifications were carried out using Metamorph software (Molecular Devices, Inc).
Zhang Y., Xie L., Gunasekar S.K., Tong D., Mishra A., Gibson W.J., Wang C., Fidler T., Marthaler B., Klingelhutz A., Abel E.D., Samuel I., Smith J.K., Cao L, & Sah R. (2017). SWELL1 is a regulator of adipocyte size, insulin signaling and glucose homeostasis. Nature cell biology, 19(5), 504-517.
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3

Lipid Quantification by AdipoRed Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lipid content was quantified using AdipoRed assay reagent (Lonza). In brief, differentiated cells were washed with PBS (pH7.4) three times, and incubated with AdipoRed solution at room temperature for 10 minutes. Cells were again washed gently with PBS. Fluorescence signal was measured with an excitation wavelength of 485 nm and an emission wavelength of 572 nm. Images were captured using an ORCA-Flash4.0 HAMAMATSU digital camera C11440 (HAMAMATSU, Japan). Fluorescence intensity quantifications were carried out using Metamorph software (Molecular Devices, Inc).
Zhang Y., Xie L., Gunasekar S.K., Tong D., Mishra A., Gibson W.J., Wang C., Fidler T., Marthaler B., Klingelhutz A., Abel E.D., Samuel I., Smith J.K., Cao L, & Sah R. (2017). SWELL1 is a regulator of adipocyte size, insulin signaling and glucose homeostasis. Nature cell biology, 19(5), 504-517.
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4

Measuring Mature Adipocyte Size

Check if the same lab product or an alternative is used in the 5 most similar protocols
For mature adipocyte size measurement, coverslips with adipocytes attached were transferred to a bath chamber on the stage of an inverted microscope (IX73, Olympus) and visualized using a 20x or 40x objective lens (Olympus). Cell images were captured using an ORCA-Flash4.0 HAMAMATSU digital camera C11440 (HAMAMATSU, Japan) and recorded using Metamorph software (Molecular Devices, Inc.). The cross-sectional area was measured as an indicator of the cell size, using Metamorph software.
Zhang Y., Xie L., Gunasekar S.K., Tong D., Mishra A., Gibson W.J., Wang C., Fidler T., Marthaler B., Klingelhutz A., Abel E.D., Samuel I., Smith J.K., Cao L, & Sah R. (2017). SWELL1 is a regulator of adipocyte size, insulin signaling and glucose homeostasis. Nature cell biology, 19(5), 504-517.
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