Cell pellets were lysed in 2x Laemmli buffer (Alfa Aesar) and performed as described previously12 (link) with the following antibodies: anti-PTEN (A2B1, Santa Cruz Biotechnology, sc-7974, 1:1000), anti-GAPDH (V-18, Santa Cruz Biotechnology, sc-20357, 1:500), anti-P65 (L8F6, Cell Signaling Technology, 6956, 1:1000), and anti-phospho-P65 Ser536 (93H1, Cell Signaling Technology, 3033, 1:1000). As secondary antibodies anti-mouse IRDye 800 (1:5000; Li-Cor Biosciences, Cat# 926-68072), anti-rabbit IRDye 680 (1:5000; Li-Cor Biosciences, Cat# 926–6807), and anti-goat IRDye 680 (1:5000; Li-Cor Biosciences, Cat# 926-32224) were used. The blot was analyzed by Odyssey CLx imaging system (Li-Cor).
Anti phospho p65 ser536 93h1
Manufactured by Cell Signaling Technology
Anti-phospho-P65 Ser536 (93H1) is a rabbit monoclonal antibody that recognizes the phosphorylated form of the p65 subunit of NF-κB at serine 536. This antibody is useful for detecting the activated form of NF-κB in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey clx imaging system, by LI COR (1 mentions)
Laemmli buffer, by Thermo Fisher Scientific (1 mentions)
Anti pten a2b1, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit irdye 680, by LI COR (1 mentions)
Anti mouse irdye 800, by LI COR (1 mentions)
Anti gapdh v18, by Santa Cruz Biotechnology (1 mentions)
Hoechst 33342 trihydrochloride trihydrate, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Anti p stat3, by Cell Signaling Technology (1 mentions)
Anti phospho iκbα ser32 36 5a5, by Cell Signaling Technology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Alexa fluor 546 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Anti p65 c 20, by Santa Cruz Biotechnology (1 mentions)
Anti c myc, by Merck Group (1 mentions)
Anti iκbα, by Cell Signaling Technology (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
2 protocols using anti phospho p65 ser536 93h1
1
Western Blot Analysis of Cell Signaling
2
Antibody Toolkit for Cellular Signaling
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The following antibodies were used: anti-GGT1 (ab88864 for western blotting, Abcam), anti-GTF2I-C-terminal (ab135619 for immunohistochemistry, Abcam), anti-p65 (C-20, Santa Cruz), anti-phospho-p65 (Ser536 93H1, Cell Signaling Technology), anti-IκBα (Cell Signaling Technology), anti-phospho-IκBα (Ser32/36 5A5, Cell Signaling Technology), anti-STAT3 (Cell Signaling Technology), anti-p-STAT3 (Cell Signaling Technology), anti-FLAG M2 (Sigma-Aldrich), anti-c-Myc (Sigma-Aldrich), Alexa Fluor 488 goat anti-rabbit IgG (H + L) (Invitrogen), Alexa Fluor 546 goat anti-mouse IgG (H + L) (Invitrogen) and Hoechst 33,342 trihydrochloride trihydrate (Life Technologies).
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