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C57bl 6 mice

Manufactured by Macrogen
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C57BL/6 mice are a commonly used inbred mouse strain. They are widely utilized in biomedical research for various applications.

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Lab products found in correlation

Bleomycin, by Santa Cruz Biotechnology (1 mentions) Wild type c57bl 6 mice, by Orient Bio (1 mentions)

2 protocols using c57bl 6 mice

1

Generation and Validation of TM4SF5 Transgenic Mice

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TM4SF5 transgenic C57BL/6 mice were generated (Macrogen, Seoul, Korea), and TM4SF5 expression was confirmed (37 (link)). The pcDNA3-hTM4SF5-FLAG plasmid (digested with NruI and DraIII), consisting of the cytomegalovirus promoter, whole human TM4SF5-FLAG sequence, and bovine growth hormone polyadenylation region, was used for the generation of C57BL/6 mice according to standard procedures, as explained previously (37 (link)). ApcMin/+ mice were kindly gifted by Dr. Young-Yun Kong (Department of Biological Sciences, Seoul National University, Seoul, Republic of Korea). ApcMin/+ mice were interbred with TgTM4SF5 to generate ApcMin/+:TgTM4SF5 offspring. All animal experiments were performed in accordance with the guidelines of the Seoul National University Institutional Animal Care and Use Committee (SNU-130225-9-2).
Lee J., Kim E., Kang M.K., Ryu J., Kim J.E., Shin E.A., Pinanga Y., Pyo K.H., Lee H., Lee E.H., Cho H., Cheon J., Kim W., Jho E.H., Kim S, & Lee J.W. (2022). Systemic TM4SF5 overexpression in ApcMin/+ mice promotes hepatic portal hypertension associated with fibrosis. BMB Reports, 55(12), 609-614.
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2

Bleomycin-induced Pulmonary Fibrosis Model

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Wild-type C57BL/6 mice (4 weeks old) were purchased from Orient Bio Inc. (Seongnam, South Korea). Global TM4SF5-knockout C57BL/6 mice (Tm4sf5−/−) were generated by Macrogen (Seoul, South Korea). Briefly, base pairs of genomic TM4SF5 surrounding exon 1 were deleted using the RGEN/Cas9 genetic scissor. The genotypes of both wild-type and Tm4sf5−/− mice were determined by PCR analysis of genomic DNA obtained from tail clippings from 3-week-old animals. Male wild-type and Tm4sf5−/− mice at 8 weeks old were treated with either saline vehicle for the control or bleomycin (1 mg/kg, Santa Cruz Biotechnology) by intratracheal injection on day 0. Mice were sacrificed on day 28 for histological analysis and hydroxyproline assay.
Kim J.E., Kim H.J., Jung J.W., Song D.G., Park D., Lee H., Um H., Park J., Nam S.H., Cho M, & Lee J.W. (2019). TM4SF5-mediated CD44v8-10 splicing variant promotes survival of type II alveolar epithelial cells during idiopathic pulmonary fibrosis. Cell Death & Disease, 10(9), 645.
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