All the products described in this protocol were available in the Hematoxylin & Eosin Stain Kit (Vector Laboratories). Stainings were performed as indicated by the manufacturer. Floating 40–50 μm brain sections were mounted on Inkjet Plus Microscope Slides (Fisher Scientific) and dried until the tissue was completely attached to the surface (1 h approximately). Slides were then rinsed in water for 5 s. Hematoxylin was applied to completely cover the tissue sections and incubated for 5 min. Slides were rinsed in two changes of distilled water (15 s each) to remove excess stain. Bluing reagent was applied to completely cover the tissue and incubated for 15 s. Slides were rinsed in 2 changes of distilled water (15 s each). Slides were dipped in 100% ethanol (10 s). Eosin Y solution was applied completely covering the tissue, incubated for 3 min, and washed in 100% ethanol for 10 s. Finally, the slide was dehydrated in 3 changes of 100% ethanol and mounted in VectaMount Mounting Medium (Vector laboratories). Images were acquired at an Olympus vs-120 microscope using a 20x objective and processed in ImageJ.
Inkjet plus microscope slides
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Inkjet Plus Microscope Slides are a specialized laboratory product designed for use with microscopes. They feature a pre-printed grid pattern that can be used as a reference for sample positioning and measurement during microscopic analysis.
Automatically generated - may contain errors
3 protocols using inkjet plus microscope slides
1
Hematoxylin and Eosin Staining of Brain Sections
2
Hematoxylin and Eosin Staining of Brain Sections
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All the products described in this protocol were available in the Hematoxylin & Eosin Stain Kit (Vector Laboratories). Stainings were performed as indicated by the manufacturer. Floating 40–50 μm brain sections were mounted on Inkjet Plus Microscope Slides (Fisher Scientific) and dried until the tissue was completely attached to the surface (1 h approximately). Slides were then rinsed in water for 5 s. Hematoxylin was applied to completely cover the tissue sections and incubated for 5 min. Slides were rinsed in two changes of distilled water (15 s each) to remove excess stain. Bluing reagent was applied to completely cover the tissue and incubated for 15 s. Slides were rinsed in 2 changes of distilled water (15 s each). Slides were dipped in 100% ethanol (10 s). Eosin Y solution was applied completely covering the tissue, incubated for 3 min, and washed in 100% ethanol for 10 s. Finally, the slide was dehydrated in 3 changes of 100% ethanol and mounted in VectaMount Mounting Medium (Vector laboratories). Images were acquired at an Olympus vs-120 microscope using a 20x objective and processed in ImageJ.
3
Synthesis and Characterization of PGD Prepolymer
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PGD prepolymer was synthesized following the method described by Solorio et al. [27 (link)]. Briefly, PGD prepolymer was synthesized by mixing glycerol and dodecanedioic acid with a 1:1 molar ratio in a 120 °C flask under nitrogen and stirring conditions for 24 h, followed by vacuum environment at 120 °C for 24 h. The viscous prepolymer was then cast onto InkJet Plus microscope slides (Fisherbrand, Pittsburgh, PA, USA) or into 3 mL glass vials (Supelco, Bellefonte, PA, USA). The PGD prepolymer spontaneously spread to a film on the microscope slides or created a flat surface on the bottom of glass vials, and was then cured at 130 °C for 48 h. A vacuum was pulled and maintained at 90 mTorrs for the duration of the curing process. All the PGD samples were soaked in the growth media for 2 days with three media changes to remove any possible cytotoxic PGD byproducts that could dissolve in the media.
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