Annexin 5 fitc pi apoptosis double staining kit

Human peritoneal mesothelial cells (HMrSV5) purchased from BNCC, Beijing, China. All cells were cultured in Dulbecco's modified eagle medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated with 5% CO₂ at 37°C. The Hangzhou Yingjian Biological Co., Ltd. developed sodium alginate (SALG) hydrogel, which is primarily made of sodium alginate and dextran. Medical Chitosan gel (Shijiazhuang Yishengtang Medical Products Co., Ltd, China), FBS (Gibco, USA), penicillin-streptomycin combined antibiotics (Gibco, USA), High Glucose DMEM (Gibco, USA), CCK-8 kit (Solarbio, China), PBS (Hyclone, USA), ROS reactive oxygen kit (Nanjing Jiancheng Institute of Biological Engineering, China), Propidium iodide dye kit (Keyueda, China) and Annexin V-FITC/PI apoptosis double-staining kit (BD, USA).

Flow cytometry was used to detect the effect of HPV16/18 E6/E7 and IGF2BP2 on cell apoptosis and the cell cycle in HeLa and SiHa cells transfected with siRNAs or plasmids. Cell apoptosis was assessed using an Annexin V-FITC/PI apoptosis double staining kit while the cell cycle was analyzed using PI/RNase staining buffer (BD, USA) according to the manufacturers' instructions. NovoExpress software (ACEA Biosciences, USA) was used for data acquisition and analysis.