For protein molar mass determination, purified SHLD3s–REV7, SHLD3 (1 to 58)–REV7, and SHLD2.3–REV7 proteins were analyzed using an ÄKTA-MALS system. A mini DAWN TREOS multiangle light scattering detector (Wyatt Technology) and an Optilab T-rEX refractometer (Wyatt Technology) were used in-line with a Superdex200 10/300 gel filtration column (GE Healthcare) preequilibrated in the buffer (20 mM Tris⋅HCl [pH 7.5], 150 mM NaCl, and 2 mM DTT) at a flow rate of 0.2 mL/min. Separation and ultraviolet (UV) detection were performed by ÄKTA Pure System (GE Healthcare), light scattering was monitored by the mini DAWN TREOS system, and concentration was measured by the Optilab TrEX differential refractometer. Molar masses of proteins were calculated using the Astra 6.1 program (Wyatt Technology) with a dn/dc value (refractive index increment) of 0.185 mL/g. The data were plotted using Prime8 software (GraphPad).
Optilab t rex differential refractometer
Manufactured by GE Healthcare
The Optilab T-rEX differential refractometer is a laboratory instrument used for precise refractive index measurements. It operates on the principle of differential refraction to determine the refractive index of liquid samples. The device provides accurate and reliable data for applications that require precise refractive index analysis.
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Lab products found in correlation
Optilab t rex refractometer, by Wyatt Technology (3 mentions)
Superdex 200 10 300 gel filtration column, by GE Healthcare (3 mentions)
Minidawn treos multi angle light scattering detector, by Wyatt Technology (3 mentions)
Minidawn treos system, by GE Healthcare (3 mentions)
Kta pure system, by GE Healthcare (3 mentions)
Astra 6.1 program, by Wyatt Technology (3 mentions)
3 protocols using optilab t rex differential refractometer
1
Protein Molar Mass Determination by MALS
2
Protein Molar Mass Determination by MALS
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For protein molar mass determination, purified Card1 proteins in buffer B with 1 mM cA4 were analyzed using an ÄKTA-MALS system. A miniDAWN TREOS multi-angle light scattering detector (Wyatt Technology) and an Optilab T-rEX refractometer (Wyatt Technology) were used in-line with Superdex200 10/300 gel filtration column (GE Healthcare) pre-equilibrated in buffer B at a flow rate of 0.2 mL/min. Separation and ultraviolet detection were performed by ÄKTA Pure system (GE Healthcare), light scattering was monitored by miniDAWN TREOS system, and concentration was measured by the Optilab T-rEX differential refractometer. Molar masses of proteins were calculated using the Astra 6.1 program (Wyatt Technology) with a dn/dc value of 0.185 mL/g.
3
Protein Molar Mass Determination by MALS
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