Ho 1 elisa kit

The ISO was procured from Sigma (St. Louis, MO, USA). The TAX was purchased from Biosynth Carbosynth (Berkshire, UK). A Cardiac troponin I (cTnI) ELISA kit was obtained from Kamiya (Tukwila, WA, USA). Kits for creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) activities were procured from Spinreact (Girona, Spain). A HO-1 ELISA kit was obtained from MyBioSource (San Diego, CA, USA). The ELISA kits for tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were supplied by R&D Systems (Minneapolis, MN, USA). Anti-NF-κB p65, anti-caspase-3, and anti-Nrf2 antibodies were obtained from ThermoFisher (Waltham, MA, USA; 1:100 dilution), while anti-Bax and anti-Bcl-2 antibodies were supplied by Abcam (Cambridge, MA, USA; 1:100 dilution).

All kits used for this part were rat-specific. MDA ELISA kit for rats was used to measure levels of malondialdehyde (MDA) in the liver homogenates (#MBS268427, MyBioSource, CA, USA). GSH ELISA kit (#MBS265966; MyBioSource, CA, USA), SOD ELISA kit (#MBS036924, MyBioSource, CA, USA), CAT ELISA kit (#MBS726781, MyBioSource, CA, USA), IL-6 ELISA kit (#MBS269892, MyBioSource, CA, USA), HO-1 ELISA kit (MBS764989, MyBioSource, CA, USA) and TNF-αELISA kits (#MBS2507393, MyBioSource, CA, USA) were used to measure the levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the liver homogenates. G6PC ELISA kit (#MBS097902 MyBioSource, CA, USA), FBP1 ELISA kit (#MBS931493, MyBioSource, CA, USA), and GLY ELISA kit (#MBS1600418; MyBioSource, CA, USA) were used to measure the levels of glucose-6 phosphatase (G-6-Pase), fructose-1,6-bisphosphatase 1 (PBP1), and glycogen in the liver homogenates. The TransAM Nrf2, NF-E2-related factor 2 (Nrf2 DNA binding ELISA) kit (#50296, ActiveMotif, Tokyo, Japan) and TransAM NF-κB p65 ELISA kits (#40096, ActiveMotif, Tokyo, Japan) were used to measure the total and cytoplasmic levels of Nrf2 and NF-κB p65 in the homogenates. All procedures were conducted according to each manufacturer’s instructions for 8 samples/groups.