Cell culture reagents and chemicals were from Sigma Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA) unless otherwise stated. Drugs and biologics as follows: ABT-737 (Selleck Chemicals); CHX (Sigma); YOYO3 and SYTO reagents (Life Technologies/Thermo Fisher Scientific), mTNFα and mTRAIL (Peprotech, Rocky Hill, NJ, USA); zVAD-fmk (ApexBio, Houston, TX, USA), birinapant (MedChem Express). Fluorophores: FITC (Cat. No. 46425, Thermo Fisher Scientific), Alexa Fluor 488 5-SDP Ester (Cat. No. A30052, Thermo Fisher Scientific), Alexa Fluor 594 NHS Ester (Cat. No. A37572, Thermo Fisher Scientific).
Mtnfα and mtrail
Manufactured by Thermo Fisher Scientific
Sourced in United States
MTNFα and mTRAIL are recombinant proteins produced by Thermo Fisher Scientific. MTNFα is a tumor necrosis factor-alpha variant, and mTRAIL is a murine tumor necrosis factor-related apoptosis-inducing ligand. These proteins are intended for use in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Z vad fmk, by Apexbio (2 mentions)
Alexa fluor 594 nhs ester, by Thermo Fisher Scientific (2 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Alexa fluor 488 5 sdp ester, by Thermo Fisher Scientific (1 mentions)
Birinapant, by MedChemExpress (1 mentions)
Abt 737, by Selleck Chemicals (1 mentions)
Dyecycle green, by Thermo Fisher Scientific (1 mentions)
Dylight 633 nhs ester, by Thermo Fisher Scientific (1 mentions)
Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (1 mentions)
Yoyo 3, by Thermo Fisher Scientific (1 mentions)
Celltrace cfse, by Thermo Fisher Scientific (1 mentions)
Dye removal column, by Thermo Fisher Scientific (1 mentions)
Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions)
Draq7, by Abcam (1 mentions)
Abt 737, by Abbott (1 mentions)
2 protocols using mtnfα and mtrail
1
Cell Culture Reagents and Compounds
2
Annexin V Fluorescent Labeling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell culture reagents and chemicals were from Sigma Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA) unless otherwise stated. Drugs and biologics as follows: ABT-737 (Abbott Pharmaceuticals, Lake Bluff, IL, USA); Tn, Tg, CHX and STS (Sigma); DRAQ7 (Abcam, Cambridge, UK); YOYO3, CellTrace CFSE, DyeCycle Green and CellEvent Caspase-3/-7 Green Detection Reagent (Thermo Fisher Scientific); mTNFα and mTRAIL (Peprotech, Rocky Hill, NJ, USA); zVAD-fmk (ApexBio, Houston, TX, USA). Recombinant Annexin V was purified, labelled with FITC, DyLight 633 NHS ester (Thermo Fisher Scientific), or AlexaFluor 594 NHS ester (Thermo Fisher Scientific), and re-purified using Dye Removal Columns (Thermo Fisher Scientific) following conjugation. Purity, stability and fluorescence of labelled recombinant proteins were analysed by SDS-PAGE. Fluorescent imaging of labelled recombinant Annexin V was accomplished using an MP ChemiDoc Imager equipped with LED excitation cubes (Bio-Rad, Hercules, CA, USA).
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