Anti mouse il 12 clone c17.8

Purified anti-mouse GM-CSF (clone MP1-22E9) and anti-mouse IL-12 (clone C17.8) were from BioXcell and used for affinity chromatography purification of GPI-GM-CSF and GPI-IL-12, respectively. GPI-GM-CSF and GPI-IL-12 were expressed in CHO-S cells and purified using affinity column chromatography as described in our earlier study [21 (link)].

Purified anti-mouse GM-CSF (clone MP1-22E9) and anti-mouse IL-12 (clone C17.8) mAbs were obtained from BioXcell and used for affinity chromatography purification of GPI-RBD-GM-CSF fusion protein and GPI-IL-12, respectively. Anti-RBD mAb (clone MM57) was obtained from Sino Biologicals (Cat#40592). FITC-conjugated goat secondary antibody against mouse IgG/IgM was purchased from BD Pharmingen (Cat# 555988). Peroxidase (HRP)-conjugated goat anti-mouse IgG F(ab’)2 specific antibody was from ThermoFisher Scientific/Pierce (Cat#31436). The antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060). Human COVID-19 convalescent serum samples were purchased from Ray Biotech (Atlanta, GA, USA). HRP-conjugated donkey anti-human IgG antibody was obtained from Jackson Immunoresearch (Cat#709-036-098). Biotinylated human ACE2 from ACRO Biosystems (Cat#AC2-H82F9) and purified RBD protein from Ray Biotech (Cat#230-30162) were purchased.