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Anti cd39

Manufactured by BD
Sourced in United States

Anti-CD39 is a monoclonal antibody used for the detection and quantification of CD39, a cell surface molecule expressed on various cell types. It is a useful tool for research applications involving the study of CD39 and its role in immunological and physiological processes.

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2 protocols using anti cd39

1

Multiparametric T cell immunophenotyping

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Single cell suspensions of T cells were stained as described [17 (link)] at 4°C with fluorochrome-labeled anti-CD3, anti-CD4, anti-CD25, anti-CD27-PE, anti-CD39, anti-CD44, anti-CD45RA, anti-CD62L, anti-CD127, anti-CXCR3, anti-CCR7 (BD PharMingen, Franklin Lakes, NJ, or BioLegend, San Diego, CA). Intracellular Foxp3, cytotoxic T lymphocyte Ag-4 (CTLA-4), Helios, T-bet, IL-2, IL-17 and IFN-γ were stained using an eBioscience Foxp3 Staining kit, according to the manufacturer’s instructions. Data were acquired on a LSR II or LSR Fortessa (BD Bioscience) and analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
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2

Immunophenotyping of CD45+, CD39+, and CD73+ cells

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SVCs were stained with anti-CD45PAN (BioLegend, San Diego, CA, USA), anti-CD39 (BD PharMingen, San Diego, CA, USA), and anti-CD73 (BD PharMingen) antibodies. Isotype-matched mAb were used as controls. Data were acquired by use of a FACSAria II (BD Biosciences, San Jose, CA, USA) and analyzed with FlowJo software. Statistical analyses were based on at least 100,000 events gated on the population of interest.
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