The levels of Aβ40 and Aβ42 were determined by electrochemiluminescence (ECL) assay using 96-well MULTI-ARRAY Multiplex Kits (Cat# K15199E-2, Meso Scale Diagnostics, Gaithersburg, MD). The TBS- and guanidine-soluble fractions of brain lysates were processed according to the manufacturer’s instructions. Aβ levels were measured using a SECTOR S 600 reader (Meso Scale Diagnostics).
Multi array multiplex kits
Manufactured by Mesoscale
Sourced in United States
The MULTI-ARRAY Multiplex Kits are a line of laboratory equipment designed for the simultaneous detection and quantification of multiple analytes in a single sample. The kits leverage multiplex assay technology to enable high-throughput analysis, streamlining research workflows.
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Lab products found in correlation
2 protocols using multi array multiplex kits
1
Quantifying Amyloid-Beta Levels
2
Multiplexed Quantification of Amyloid-Beta
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96-well and 384-well MULTI-ARRAY Multiplex Kits (Meso Scale Discovery, Gaithersburg, MD, USA) were used to measure the level of Aβ38, Aβ40, Aβ42 and sAPPβ, by electrochemiluminescence (ECL) assay. Supernatants of BV2 cells after the Aβ clearance assay or brain homogenates TBS- and SDS-soluble fractions were further processed according to the manufacturer’s instructions. sAPPβ and Aβ species peptides were detected with a monoclonal antibody and quantified by using a SECTOR Imager 6000 reader (Meso Scale Discovery). Values were normalized to scrambled control or to WT, for in vitro and in vivo experiments, respectively.
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